Optimization of culture conditions and scale-up to pilot and plant scales for vancomycin production by Amycolatopsis orientalis

Applied Microbiology and Biotechnology
Hyung-Moo JungJung-Kul Lee

Abstract

This report describes the optimization of culture conditions for vancomycin production by Amycolatopsis orientalis KCCM-10836P, an identified high-vancomycin-producing strain (US11/712,494). Among the conditions tested, pH and the dissolved oxygen tension (DOT) were key factors affecting vancomycin production. When the pH and DOT were controlled at 7.0 and 20-30%, respectively, a dry-cell weight (DCW) of 62.0 g l(-1) and a vancomycin production of 11.5 g l(-1) were obtained after 120 h of batch culture, corresponding to a specific vancomycin content of 185.4 mg g-DCW(-1). Vancomycin production was scaled up from a laboratory scale (7-l fermentor) to a pilot scale (300 l) and a plant scale (5,000 l) using the impeller tip velocity (V (tip)) as a scale-up parameter. Vancomycin production at the laboratory scale was similar to those at the pilot and plant scales.

References

Jun 1, 1978·Annals of Internal Medicine·F V Cook, W E Farrar
Apr 1, 1991·Antimicrobial Agents and Chemotherapy·R Nagarajan
Feb 1, 1973·Canadian Journal of Microbiology·F P Mertz, L E Doolin
Jan 1, 1984·Annual Review of Microbiology·J C Barna, D H Williams
Feb 1, 1983·The Journal of Infectious Diseases·C W Norden, M Shaffer
Dec 1, 1981·Antimicrobial Agents and Chemotherapy·M S EngineerG P Bodey
Nov 1, 1981·Reviews of Infectious Diseases·B A CunhaC H Nightingale
Apr 1, 1999·Biotechnology and Bioengineering·J J McIntyreA T Bull
Mar 20, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·István PintérJudit Nagy

Citations

May 25, 2012·Journal of the American Chemical Society·Brandon S FowlerScott J Miller
Nov 28, 2009·The Science of the Total Environment·Celia Ponder, Michael Overcash
Feb 11, 2009·Biotechnology Progress·Beth JunkerNeal Connors
Oct 8, 2016·Organic & Biomolecular Chemistry·Binuraj R K MenonJason Micklefield
Jun 6, 2019·BMC Biotechnology·Hong GaoMargaret C M Smith
Dec 18, 2020·Frontiers in Plant Science·Katharine DavisSarah E O'Connor

Related Concepts

Coryneform Group
Fermentation
Hydrogen-Ion Concentration
Industrial Microbiology
Pilot Projects
AB-Vancomycin

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