Optimization of culture conditions for the derivation and propagation of baboon (Papio anubis) induced pluripotent stem cells

PloS One
Christopher S NavaraJohn R McCarrey

Abstract

Induced pluripotent stem cells (iPSCs) offer the possibility of cell replacement therapies using patient-matched cells to treat otherwise intractable diseases and debilitations. To successfully realize this potential, several factors must be optimized including i) selection of the appropriate cell type and numbers to transplant, ii) determination of the means of transplantation and the location into which the transplanted cells should be delivered, and iii) demonstration of the safety and efficacy of the cell replacement protocol to mitigate each targeted disease state. A majority of diseases or debilitations likely to be targeted by cell-based therapeutic approaches represent complex conditions or physiologies manifest predominantly in primates including humans. Nonhuman primates afford the most clinically relevant model system for biomedical studies and testing of cell-based therapies. Baboons have 92% genomic similarity with humans overall and especially significant similarities in their immunogenetic system, rendering this species a particularly valuable model for testing procedures involving cell transplants into living individuals. To maximize the utility of the baboon model, standardized protocols must be developed for t...Continue Reading

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Citations

Feb 29, 2020·Primate Biology·Ignacio Rodriguez-PoloRüdiger Behr
Dec 7, 2019·Brain Research·Mohammed A Mostajo-RadjiAlex A Pollen
Feb 12, 2021·Scientific Reports·Johanna GeuderWolfgang Enard
Apr 21, 2021·The FEBS Journal·Michael Dannemann, Irene Gallego Romero

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BETA
blood draw
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REST
Biosystems RQ manager
Applied
Pluristem
ImageJ

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