Optimization of DNA immobilization on gold electrodes for label-free detection by electrochemical impedance spectroscopy

Biosensors & Bioelectronics
Simon D KeighleyP Migliorato

Abstract

The ability to immobilize DNA probes onto gold substrates at an optimum surface density is key in the development of a wide range of DNA biosensors. We present a method to accurately control probe DNA surface density by the simultaneous co-immobilization of thiol modified probes and mercaptohexanol. Probe surface density is controlled by the thiol molar ratio in solution, with a linear relationship between thiol molar ratio and probe density spanning (1-9) x10(12)/cm2. The probe surface density per microscopic surface area was determined using chronocoulometry, and a detailed analysis of the method presented. Using this sample preparation method, the effect of probe density and hybridization on the charge transfer resistance with the negatively charged ferri/ferrocyanide redox couple was determined. Above a threshold probe surface density of 2.5 x 10(12)/cm2, electrostatic repulsion from the negatively charged DNA modulates the charge transfer resistance, allowing hybridization to be detected. Below the threshold density no change in charge transfer resistance with probe density or with hybridization occurs. The probe surface density was optimized to obtain the maximum percentage change in charge transfer resistance with hybrid...Continue Reading

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Citations

Mar 13, 2009·Analytical and Bioanalytical Chemistry·Julie M Goddard, David Erickson
Mar 1, 2012·Chemical Reviews·Emil Paleček, Martin Bartošík
Sep 6, 2008·Biosensors & Bioelectronics·Simon D KeighleyPiero Migliorato
Mar 27, 2016·Biosensors & Bioelectronics·Grace HenihanTill T Bachmann
Jun 25, 2015·Biointerphases·Igor LevchenkoKostya Ken Ostrikov
Dec 27, 2011·Journal of Colloid and Interface Science·Bradley W BiggsAndrea M Armani
Aug 27, 2015·Scientific Reports·Jinghua FangKostya Ken Ostrikov
Mar 16, 2017·Scientific Reports·N AliakbarinodehiS Carrara
Nov 22, 2017·International Journal of Molecular Sciences·Peggy ReichDieter Beckmann
Aug 3, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Magdalena Gebala, Wolfgang Schuhmann
Jun 28, 2011·The Analyst·Mohtashim Hassan Shamsi, Heinz-Bernhard Kraatz
Aug 2, 2017·Nanotechnology·Andrea MescolaAthanassia Athanassiou
Oct 4, 2017·Biosensors·Meiirbek IslamovLuis Rojas-Solórzano
Nov 30, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Evgeniy MervinetskyShlomo Yitzchaik
Sep 22, 2019·Sensors·José R EspinosaSergio M Durón
Jun 26, 2020·Food Science and Biotechnology·Changhoon Chai, Se-Wook Oh
Dec 5, 2017·Analytical and Bioanalytical Chemistry·Nicole BahnerDieter Beckmann
Jan 4, 2012·Chemistry : a European Journal·Alessandra BonanniMartin Pumera
Apr 29, 2016·Nanotechnology·L FradetalV Stambouli
Jul 2, 2016·Essays in Biochemistry·Jules L HammondJan Tkac

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