Optimization of ethylene glycol production from (D)-xylose via a synthetic pathway implemented in Escherichia coli

Microbial Cell Factories
Ceren AlkimThomas Walther

Abstract

Ethylene glycol (EG) is a bulk chemical that is mainly used as an anti-freezing agent and a raw material in the synthesis of plastics. Production of commercial EG currently exclusively relies on chemical synthesis using fossil resources. Biochemical production of ethylene glycol from renewable resources may be more sustainable. Herein, a synthetic pathway is described that produces EG in Escherichia coli through the action of (D)-xylose isomerase, (D)-xylulose-1-kinase, (D)-xylulose-1-phosphate aldolase, and glycolaldehyde reductase. These reactions were successively catalyzed by the endogenous xylose isomerase (XylA), the heterologously expressed human hexokinase (Khk-C) and aldolase (Aldo-B), and an endogenous glycolaldehyde reductase activity, respectively, which we showed to be encoded by yqhD. The production strain was optimized by deleting the genes encoding for (D)-xylulose-5 kinase (xylB) and glycolaldehyde dehydrogenase (aldA), and by overexpressing the candidate glycolaldehyde reductases YqhD, GldA, and FucO. The strain overproducing FucO was the best EG producer reaching a molar yield of 0.94 in shake flasks, and accumulating 20 g/L EG with a molar yield and productivity of 0.91 and 0.37 g/(L.h), respectively, in a c...Continue Reading

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Citations

Jun 24, 2016·PloS One·Kulika ChomvongJamie H D Cate
Oct 19, 2017·Applied Microbiology and Biotechnology·Laura SalusjärviLaura Ruohonen
May 19, 2019·Genes·Manuel SalvadorJose I Jimenez
Apr 16, 2020·Microbial Cell Factories·Zhongxi ZhangJian Hao
Jul 20, 2020·Biotechnology and Applied Biochemistry·Xiyang LuJian Hao
Feb 6, 2020·Biotechnology for Biofuels·Zhe ZhaoGuang Zhao
Feb 2, 2019·Applied Microbiology and Biotechnology·Laura SalusjärviMervi Toivari
Jan 24, 2021·Microbial Cell Factories·Aditya Vikram PanditRadhakrishnan Mahadevan
Feb 11, 2021·Critical Reviews in Biotechnology·Angelo B BañaresWook-Jin Chung
Mar 13, 2020·Biotechnology Journal·Yu Jung SohnSi Jae Park
Apr 24, 2017·Metabolic Engineering·M Ahsanul IslamGregory Stephanopoulos
Oct 1, 2018·Metabolic Engineering·Boonsom UranukulGregory Stephanopoulos
Jul 4, 2021·Applied Microbiology and Biotechnology·Angelo B BañaresWook-Jin Chung

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Methods Mentioned

BETA
Feature Extraction
PCR
gene knock-outs

Software Mentioned

Feature Extraction

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