Optimization of fibronectin-assisted retroviral gene transfer into human CD34+ hematopoietic cells

Human Gene Therapy
H HanenbergD A Williams

Abstract

Efficient retroviral gene transfer into hematopoietic stem and progenitor cells can be achieved by co-localizing retrovirus and target cells on specific adhesion domains of recombinant fibronectin (FN) fragments. In this paper, we further optimize this technology for human CD34+ cells. Investigating the role of cytokine prestimulation in retrovirus-mediated gene transfer on plates coated with the recombinant FN CH-296 revealed that prestimulation of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood (PB) CD34+ cells was essential to achieve efficient gene transfer into clonogenic cells. The highest gene transfer occurred by prestimulating PB CD34+ cells for 40 hr with a combination of stem cell factor (SCF), G-CSF, and megakaryocyte growth and development factor (MGDF) prior to retroviral infection on CH-296. Surprisingly, a prolonged simultaneous exposure of primary CD34+ PB cells to retrovirus and cytokines in the presence of CH-296 lowered the gene transfer efficiency. Gene transfer into cytokine prestimulated CD34+ bone marrow (BM) cells was not influenced by increasing the coating concentrations of a recombinant FN fragment, CH-296, nor was it adversely influenced by increasing the number of CD34+ tar...Continue Reading

References

May 8, 1992·Science·W F Anderson
Jun 11, 1992·Nature·A D Miller
Aug 1, 1986·Molecular and Cellular Biology·A D Miller, C Buttimore
Feb 1, 1995·Current Opinion in Pediatrics·D B Kohn
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·D G MillerA D Miller
Feb 11, 1993·Nucleic Acids Research·V A MalkovM D Frank-Kamenetskii
May 14, 1993·Science·R C Mulligan
Aug 1, 1995·Journal of Virological Methods·A B BahnsonJ A Barranger
Feb 1, 1996·British Journal of Haematology·A LeitnerG Fritsch
Sep 1, 1996·Trends in Microbiology·R E Bachelder, N L Letvin
Sep 10, 1996·Human Gene Therapy·V W Van Beusechem, D Valerio
May 1, 1997·Australian and New Zealand Journal of Ophthalmology·B Kumar, G J Crawford

❮ Previous
Next ❯

Citations

May 6, 2003·Experimental Cell Research·Thalia Romani de WitJan A van Mourik
Nov 27, 1999·Leukemia Research·M FlasshoveS Seeber
May 29, 2002·Experimental Hematology·Helmut HanenbergArleen D Auerbach
Jun 6, 2009·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Tonya S Rans, Ronald England
Apr 20, 2010·Nature Genetics·Fiona VazChristopher G Mathew
Dec 14, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Patrick F KellyDavid A Williams
Oct 25, 2000·Human Gene Therapy·D A Williams, F O Smith
May 3, 2000·Journal of Hematotherapy & Stem Cell Research·J S ReeseS L Gerson
Oct 31, 2000·Current Opinion in Hematology·M SadelainI Rivière
Dec 21, 2004·British Journal of Haematology·Amit C NathwaniDavid C Linch
Jun 17, 2010·The Journal of Clinical Investigation·Brian C BeardHans-Peter Kiem

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.