Optimization of glucose oxidase production by Aspergillus niger using genetic- and process-engineering techniques

Applied Microbiology and Biotechnology
K HellmuthU Rinas

Abstract

Wild-type Aspergillus niger NRRL-3 was transformed with multiple copies of the glucose oxidase structural gene (god). The gene was placed under the control of the gpdA promoter of A. nidulans. For more efficient secretion the alpha-amylase signal peptide from A. oryzae was inserted in front of god. Compared to the wild type, the recombinant strain NRRL-3 (GOD3-18) produced up to four times more extracellular glucose oxidase under identical culture conditions. Addition of yeast extract (2 gl-1) to a mineral salts medium containing only glucose as carbon source increased volumetric and specific extracellular glucose oxidase activities by 130% and 50% respectively. With the same medium composition and inoculum size, volumetric and specific extracellular glucose oxidase activities increased more than ten times in bioreactor cultivations compared to shake-flask cultures.

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Citations

Jul 20, 1996·Biotechnology and Bioengineering·S PluschkellU Rinas
Sep 1, 2005·Applied Microbiology and Biotechnology·K G ClarkeS T L Harrison
Jun 14, 2005·Journal of Industrial Microbiology & Biotechnology·A A Khattab, W A Bazaraa
Jan 23, 2010·Microbial Cell Factories·Saul N RochaAndreas K Gombert
May 1, 1997·Journal of Applied Microbiology·A Gromada, J Fiedurek
Aug 16, 2001·Biotechnology and Bioengineering·M G WiebeP J Punt
Nov 1, 1996·Applied Microbiology and Biotechnology·D G HatzinikolaouP Stougaard
Jan 24, 2013·Journal of the American Chemical Society·Ling YanChaoyong James Yang

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