PMID: 8939627Oct 1, 1996Paper
Optimization of heterologous protein production in Escherichia coli
Current Opinion in Biotechnology
M J WeickertP O Olins
Abstract
Escherichia coli has long been the primary prokaryotic host for the synthesis of heterologous proteins. Recent advances have been made in the expression of complex proteins as soluble, functional molecules, complete with prosthetic groups, disulfide bonds, and quaternary structure. The development of alternative promoter and induction strategies has improved the options available for manipulating the expression conditions, which are frequently critical to soluble yield.
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