Optimization of in vitro vascular cell transfection with non-viral vectors for in vivo applications

The Journal of Gene Medicine
Ibrahim ElmadbouhJean-Baptiste Michel

Abstract

Syngeneic vascular cells are interesting tools for indirect gene therapy in the cardiovascular system. This study aims to optimize transfection conditions of primary cultures of vascular smooth muscle cells (VSMCs) using different non-viral vectors and zinc as an adjuvant and to implant these transfected cells in vivo. Non-liposomal cationic vectors (FuGene 6), polyethylenimines (ExGen 500), and histidylated polylysine (HPL) were used as non-viral vectors in vitro with secreted alkaline phosphatase (SEAP) as reporter gene. Transfection efficiency was compared in cultured rat, rabbit and human VSMCs and fibroblasts. Zinc chloride (ZnCl2) was added to optimize transfection of rat VSMCs in vitro which were then seeded in vivo. Much higher SEAP levels were obtained in rabbit cells with FuGene 6 (p <0.0001) at day 2 than in equivalent rat and human cells. Rat VSMCs transfected in vitro with FuGene 6 and ExGen 500 expressed higher SEAP levels than with HPL. In rat VSMCs, SEAP secretion was more than doubled by addition of 250 microM ZnCl2 (p <0.0001) for all vectors. Seeding of syngeneic VSMCs transfected under optimized conditions (FuGene 6/pcDNA3-SEAP +250 microM ZnCl2) into healthy Lewis rats using various routes or into post-infa...Continue Reading

References

Jan 1, 1992·Critical Reviews in Biotechnology·N S Yang
Jan 1, 1994·Circulation·J G PickeringJ M Isner
Feb 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·I Escargueil-BlancA Nègre-Salvayre
Jun 1, 1997·Pharmaceutical Research·H E HoflandS M Sullivan
Aug 1, 1997·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·R Urrutia
Jan 31, 1998·Cardiovascular Research·Y MaedaK Ozawa
Oct 14, 1998·The Journal of Clinical Investigation·E AllaireA W Clowes
Apr 13, 1999·Journal of Molecular and Cellular Cardiology·R K LiD A Mickle
May 29, 1999·Bioconjugate Chemistry·P Midoux, M Monsigny
Feb 16, 2000·Lancet·S Ylä-Herttuala, J F Martin
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·S ArmeanuS Nikol
Oct 4, 2000·Physiological Genomics·M RichterM D Allen
Oct 12, 2000·Cellular and Molecular Life Sciences : CMLS·I HellgrenC Sylvén
Feb 15, 2001·Gene Therapy·A LawrieC M Newman
Apr 9, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·M ImanishiY Sugiura
Apr 11, 2001·Mutation Research·I E Dreosti
Apr 26, 2001·Biophysical Chemistry·H BinderO Zschörnig
Aug 23, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·C L Gebhart, A V Kabanov
Oct 23, 2001·Journal of Vascular Surgery·D GomesJ B Michel
Dec 18, 2001·Advanced Drug Delivery Reviews·R KircheisE Wagner
Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Roger GaertnerJean-Baptiste Michel
Jul 13, 2002·Muscle & Nerve·Birgit NeuhuberCarol E Torgan
Sep 11, 2002·The Journal of Gene Medicine·Chantal PichonPatrick Midoux
Nov 19, 2002·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jaroslav PelisekSigrid Nikol
Dec 19, 2002·Biochimica Et Biophysica Acta·H FanecaM C Pedrosa de Lima
Apr 2, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Edmund J NiedzinskiMichael J Bennett
Dec 18, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L G SøndergaardJ Rungby
Feb 5, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Lorraine M WorkAndrew H Baker

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Citations

Nov 7, 2007·Molecular Medicine·Husnain K HaiderMuhammad Ashraf
Oct 29, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Keiji Itaka, Kazunori Kataoka
Feb 14, 2007·Journal of Biomedical Materials Research. Part a·Aurélie San JuanLaurent J Feldman
Jun 15, 2007·Journal of Biomedical Materials Research. Part a·Aurélie San JuanLaurent J Feldman
Apr 12, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Thomas KeanMaya Thanou
Jun 7, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Keiji ItakaKazunori Kataoka
Jul 9, 2005·American Journal of Physiology. Cell Physiology·David A Dean
Jul 14, 2007·The International Journal of Artificial Organs·I ElmadbouhJ C Chachques

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