Optimization of indigo production by a newly isolated Pseudomonas sp. QM

Journal of Basic Microbiology
Yuanyuan QuJiti Zhou

Abstract

Optimization of indigo production process from indole using a newly isolated phenol-degrading bacterial strain was performed by Plackett-Burman design and response surface methodology. The strain designated as QM was identified as Pseudomonas sp. according to 16S rDNA analysis. Spectrum analysis of indole biotransformation products revealed the presence of indigo and a by-product indirubin. To improve indigo yield, Plackett-Burman design was used to select significant factors from 8 viriables. Then response surface methodology based on a 2(3) central composite design was used to further optimize the transformation process. Under the optimal conditons, strain QM can produce 27.20 mg/l indigo after 24 h cultivation at 30 °C, which was 151.3% higher than that from the initial conversion condition. The results indicated that Pseudomonas sp. QM should be a potential candidate for indigo industial production.

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Citations

Jul 31, 2012·Journal of Bacteriology·Qiao MaPing Xu
Aug 11, 2015·Journal of Environmental Sciences (China)·Yuanyuan QuJiti Zhou
Jun 13, 2015·Genome Announcements·Yuanyuan QuPing Xu
Mar 3, 2015·Applied Biochemistry and Biotechnology·Yuanyuan QuJiti Zhou
Feb 6, 2015·Frontiers in Microbiology·Pandiyan RajeswariSolomon Robinson David Jebakumar
Jan 24, 2014·International Journal of Microbiology·Polpass Arul JoseSolomon Robinson David Jebakumar
Sep 5, 2019·Bioprocess and Biosystems Engineering·Chunxiao DaiYuanyuan Qu

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