Optimization of Jirisan Mountain Cudrania tricuspidata leaf substance extraction across solvents and temperatures.

Journal of Pharmacopuncture
Yong Ju Kim

Abstract

The aim of this study is to optimize the extraction of beneficial substance from Cudrania tricuspidata leaves grown at Jirisan Mountain in South Korea by three different solvents depending on extraction time and at different temperature. The total phenolic contents were determined by the method reported by Sánchez-Moreno et al. The total flavonoid contents were analyzed by Slinkard and Singleton. The DPPH radical scavenging activity was determined according to the method reported by Blois. The extraction yield for each solvent is 9.05-14.1%, 2.17-5.67%, and 2.3-3.9% for D.W., ethanol, and hexane, respectively. The overall results were maximized for the extract obtained with D.W. for 5 min at 100°C. The average phenol contents were 77.11, 45.64, and 0.343 mg/g at 100°C in water, 78°C in ethanol, and 68°C in hexane, respectively. The flavonoid contents were the highest in the materials extracted with D.W., and were increased with increasing temperature, regardless of the extraction solvents, whether water (green), polar organic ethanol, or nonpolar organic hexane. In the ethanol extract, the flavonoid contents are increased gradually from 5.66 mg/g to 7.73 mg/g. The total flavonoid contents were proportional to the concentrations...Continue Reading

References

Jan 20, 2005·Journal of Clinical Nursing·Sang-Dol Kim, Hee-Seung Kim
Oct 6, 2005·Bioorganic & Medicinal Chemistry Letters·Byong Won LeeKi Hun Park
Mar 25, 2011·Mini Reviews in Medicinal Chemistry·J M Calderón-MontañoM López-Lázaro
Jan 1, 2010·African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM·M I Khalil, S A Sulaiman
Jan 19, 2012·Nutrients·Rong Tsao

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