Optimization of MALDI-TOF MS for strain level differentiation of Arthrobacter isolates

Journal of Microbiological Methods
Márta VarghaCindy H Nakatsu

Abstract

Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be a rapid and sensitive method for characterization of bacteria, but it has not yet become a routine microbiological procedure. Currently there are no standardized protocols that would allow development of large libraries of reproducible protein profiles from a broad range of microorganisms to use for identification purposes. Important variables that may affect spectrum quality are MALDI matrices, solvents, cell growth condition, and culture age. In the present study our aim was to: (1) to determine optimal sample preparation and MALDI conditions for discrimination at the strain level; (2) to determine if changes in growth cycle correlated with MALDI spectrum changes; and (3) to compare level of isolate discrimination based on their MALDI spectra versus their 16S rRNA gene sequence. Using 16 strains of the Gram positive bacterium Arthrobacter, optimal spectra were obtained using two-layer sample application of intact cells grown on solid surface overlaid with a matrix consisting of sinapinic acid (SA) or alpha-cyano-hydroxy-cinnaminic acid (CHCA) in 50:50 acetonitrile:water solvent with 2% trifluoroacetic acid. Spectru...Continue Reading

References

Jun 1, 1997·FEMS Microbiology Reviews·P MessnerM Dorner
Jun 11, 1999·Analytical Chemistry·R J ArnoldJ P Reilly
Feb 9, 2002·Mass Spectrometry Reviews·C Fenselau, P A Demirev
Apr 11, 2003·Journal of the American Society for Mass Spectrometry·Tracie L WilliamsSteven M Musser
Jan 9, 2004·Applied and Environmental Microbiology·Danielle N DickinsonKasthuri Venkateswaran
Jan 13, 2004·Journal of Clinical Microbiology·Michael LefmannUlf B Göbel
Sep 21, 2004·Rapid Communications in Mass Spectrometry : RCM·Virginie RuelleEdwin De Pauw
Mar 29, 2005·Journal of the American Society for Mass Spectrometry·Sharon C WunschelEdward White

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Citations

Dec 8, 2010·Future Microbiology·Piseth SengDidier Raoult
Nov 17, 2012·Journal of Microbiological Methods·Silpak Biswas, Jean-Marc Rolain
Aug 6, 2011·Microbes and Infection·Julia LienardGilbert Greub
Jun 28, 2011·Journal of Microbiological Methods·Jonas GhyselinckPaul De Vos
Feb 8, 2011·Systematic and Applied Microbiology·Katrien De BruynePeter Vandamme
Jul 1, 2009·Proteomics. Clinical Applications·Martin von BergenUwe Roesler
Nov 6, 2007·Experimental Dermatology·Marcel ErhardJohannes Wöstemeyer
Apr 19, 2011·Mass Spectrometry Reviews·Ondrej ŠedoZbyněk Zdráhal
May 11, 2011·Mass Spectrometry Reviews·Yen-Peng Ho, P Muralidhar Reddy
Sep 22, 2012·Mass Spectrometry Reviews·Todd R SandrinStephanie Schumaker
Jun 23, 2009·International Journal of Systematic and Evolutionary Microbiology·Peter SchumannUNKNOWN Subcommittee on the Taxonomy of the Suborder Micrococcineae of the International Committee on Systematics of Prokaryotes
Nov 21, 2009·Journal of Medical Microbiology·Claire MolinerPierre-Edouard Fournier
Nov 19, 2011·FEMS Microbiology Reviews·Antony CroxattoGilbert Greub
Aug 20, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L FerreiraJ M González-Buitrago
Jul 1, 2016·Japanese Journal of Infectious Diseases·Kei YamamotoNorio Ohmagari

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