Optimization of medium composition for the production of antimicrobial activity by Bacillus subtilis B38

Biotechnology Progress
Olfa TabbeneFerid Limam

Abstract

An antimicrobial activity produced by Bacillus subtilis B38 was found to be effective against several bacteria, including pathogenic and spoilage microorganisms such as, Listeria monocytogenes, Salmonella enteridis, and clinical isolates of methicillin-resistant Staphylococcus species. Nutrients such as carbon, nitrogen sources, and inorganic salts enhanced the production level of the antibacterial activity by B. subtilis B38. A first screening step showed that lactose, ammonium succinate, and manganese most influenced both cell growth and antibacterial activity production. These three factors varied at two levels in eight experiments using full factorial design. Results indicated that maximum cell growth (OD = 10.2) and maximum production of antibacterial activity (360 AU/mL) were obtained in a modified medium containing 1.5% (w/v) lactose, 0.15% (w/v) ammonium succinate, and 0.3 mg/L manganese. Depending on the indicator strain used, the antibacterial activity was 2- to 4-fold higher in the modified culture medium than in TSB medium under the same conditions. Thin layer chromatography-bioautography assay showed the presence of three active spots with R(f) values of 0.47, 0.7, and 0.82 in TSB medium. However, the inhibition zo...Continue Reading

References

Aug 1, 1976·Antimicrobial Agents and Chemotherapy·E J Vandamme, A L Demain
Feb 1, 1977·Antimicrobial Agents and Chemotherapy·W K Williams, E Katz
Mar 1, 1987·Journal of General Microbiology·F BessonG Michel
Jul 1, 1973·Antimicrobial Agents and Chemotherapy·K Basak, S K Majumdar
Apr 1, 1974·Journal of General Microbiology·H I Haavik
Aug 1, 1995·Applied Microbiology and Biotechnology·J L MilnerJ Handelsman
Aug 1, 1996·Applied and Environmental Microbiology·J L MilnerJ Handelsman
Aug 29, 1998·Journal of Applied Microbiology·B HyronimusM C Urdaci
Aug 14, 2002·Journal of Applied Microbiology·D Bizani, Adriano Brandelli
Aug 30, 2002·Applied and Environmental Microbiology·M V Leal-SánchezJ L Ruiz-Barba
Feb 18, 2004·Applied Microbiology and Biotechnology·D Bizani, Adriano Brandelli
Apr 28, 2005·Molecular Microbiology·Torsten Stein

Citations

Feb 18, 2012·Natural Product Research·Salem ElkahouiHartmut Laatsch
Jan 28, 2014·Environmental Science and Pollution Research International·Yun KongXiangyang Xu
Jul 12, 2011·Journal of Industrial Microbiology & Biotechnology·Janeth Adriana Martínez-CardeñasJ Eleazar Barboza-Corona
Jan 15, 2010·FEMS Microbiology Letters·Olfa TabbeneFerid Limam

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.