Optimization of Phenolic Compounds Extraction from Flax Shives and Their Effect on Human Fibroblasts

Evidence-based Complementary and Alternative Medicine : ECAM
Magdalena CzemplikAnna Kulma

Abstract

The goal of this study was to evaluate the most effective technique for extraction of phenolics present in flax shives and to assess their effect on human fibroblasts. Flax shives are by-products of fibre separation, but they were found to be a rich source of phenolic compounds and thus might have application potential. It was found that the optimal procedure for extraction of phenolics was hydrolysis enhanced by the ultrasound with NaOH for 24 h at 65°C and subsequent extraction with ethyl acetate. The influence of the flax shives extract on fibroblast growth and viability was assessed using the MTT and SRB tests. Moreover, the influence of flax shives extract on the extracellular matrix remodelling process was verified. The 20% increase of the viability was observed upon flax shives extract treatment and the decrease of mRNA collagen genes, an increase of matrix metalloproteinase gene expression, and reduction in levels of interleukin 6, interleukin 10, and suppressor of cytokinin signaling 1 mRNA were observed. Alterations in MCP-1 mRNA levels were dependent on flax shives extract concentration. Thus, we suggested the possible application of flax shives extract in the wound healing process.

References

Oct 9, 1999·Clinical Microbiology Reviews·M M Cowan
Sep 26, 2000·American Journal of Physiology. Cell Physiology·L Y ZangV Vallyathan
Mar 10, 2001·Ultrasonics Sonochemistry·A Tuulmets, P Raik
Oct 31, 2002·Journal of Agricultural and Food Chemistry·Sergio Gómez-AlonsoGiuseppe Fregapane
Dec 23, 2003·Journal of Biotechnology·Magdalena WróbelJan Szopa
Aug 30, 2005·Mutation Research·M A SoobratteeT Bahorun
Sep 28, 2006·Journal of Agricultural and Food Chemistry·Jin-Woo Kim, G Mazza
Dec 25, 2007·The Korean journal of laboratory medicine·Yu Kyung KimKyung Eun Song
Jan 12, 2008·Journal of Clinical Biochemistry and Nutrition·Marimuthu SrinivasanVenugopal P Menon
Jan 8, 2010·International Journal of Molecular Sciences·Ryszard AmarowiczRonald B Pegg
Oct 27, 2010·Biomedizinische Technik. Biomedical Engineering·Tomasz GredesJan Szopa
Jul 12, 2011·Expert Reviews in Molecular Medicine·Timothy J Koh, Luisa Ann DiPietro
Oct 11, 2011·Journal of the American Academy of Dermatology·Katarzyna Skórkowska-TelichowskaJan Szopa
Nov 11, 2011·Journal of Agricultural and Food Chemistry·Jara Pérez-Jiménez, Josep Lluís Torres
May 1, 2012·Journal of Agricultural and Food Chemistry·Magdalena ZukJan Szopa
Dec 12, 2012·BMC Complementary and Alternative Medicine·Magdalena CzemplikJan Szopa
Dec 27, 2013·Indian Journal of Cancer·R B NerliM B Hiremath
Apr 11, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Dolly SinghSung Soo Han
Jul 6, 2014·Inquiry : a Journal of Medical Care Organization, Provision and Financing·Adam S Wilk
Mar 19, 2015·Advances in Wound Care·Meilang Xue, Christopher J Jackson

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Citations

Dec 11, 2020·Oxidative Medicine and Cellular Longevity·Tomasz GębarowskiKazimierz Gąsiorowski

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Methods Mentioned

BETA
genetic modification
PCR
transgenic

Software Mentioned

Statistica

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