Optimization of polyhydroxybutyrate production by mixed cultures submitted to aerobic dynamic feeding conditions

Biotechnology and Bioengineering
Luisa S SerafimM A M Reis

Abstract

Activated sludge submitted to aerobic dynamic feeding conditions showed a good and stable capacity to store polyhydroxybutyrate (PHB). The system, working for 2 years, selected a microbial population with a high PHB storage capacity. The influence of carbon and nitrogen concentrations on the PHB accumulation yield was studied in a range of 15-180 Cmmol/l for acetate and 0-2.8 Nmmol/l for ammonia. Low ammonia concentrations favored PHB accumulation. The maximum PHB content, 67.5%, was obtained for 180 Cmmol/l of acetate supplied in one pulse. However, such high substrate concentration proved to be inhibitory for the storage mechanism, causing a slowdown of the specific PHB storage rate. In order to avoid substrate inhibition, 180 Cmmol/l of acetate was supplied in different ways: continuously fed and in three pulses of 60 Cmmol/l each. In both cases the specific PHB storage rate increased and the PHB content obtained were 56.2% and 78.5%, respectively. The latter value of PHB content is similar to that obtained by pure cultures and was never reported for mixed cultures. Addition of acetate by pulses controlled by the oxygen concentration was kept for 16 days, the PHB content being always above 70% of cell dry weight.

References

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Citations

Nov 13, 2008·Applied Microbiology and Biotechnology·Luisa S SerafimMaria A M Reis
Jan 7, 2009·Applied Microbiology and Biotechnology·Meeta PatelPeter H Dare
Jun 7, 2011·Bioprocess and Biosystems Engineering·Bertan BasakBahar Kasapgil Ince
Apr 18, 2008·Applied Biochemistry and Biotechnology·Gregory R MockosDavid N Thompson
Jan 7, 2014·Applied Biochemistry and Biotechnology·Sebnem OzdemirOzer Cinar
Dec 18, 2012·Environmental Technology·Orhan InceMustafa Kolukirik
Jul 5, 2011·Applied and Environmental Microbiology·Allison J PiejaCraig S Criddle
Feb 22, 2014·Bioresource Technology·Diogo QueirósLuísa S Serafim
Jun 16, 2011·Applied Microbiology and Biotechnology·Helena Moralejo-GárateMark C M van Loosdrecht
Jul 28, 2016·Critical Reviews in Environmental Science and Technology·Oskar ModinMalte Hermansson
Oct 29, 2013·New Biotechnology·Liliana Montano-HerreraBronwyn Laycock
Jan 1, 2012·Journal of Environmental Sciences (China)·Qinxue WenNanqi Ren
Aug 28, 2012·Water Research·Yang JiangRobbert Kleerebezem
Jan 18, 2011·Journal of Environmental Sciences (China)·Qinxue WenWei Chen
Jun 9, 2009·Waste Management·Anshuman A KhardenavisTapan Chakrabarti
Mar 16, 2007·Bioresource Technology·Simon BengtssonThomas Welander
Apr 2, 2011·Biotechnology and Bioengineering·Yang JiangMark C M van Loosdrecht
Jul 10, 2013·Biotechnology and Bioengineering·Helena Moralejo-GárateMark C M van Loosdrecht
Oct 26, 2010·International Journal of Systematic and Evolutionary Microbiology·Yang JiangMark van Loosdrecht

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