Optimization of probiotic and lactic acid production by Lactobacillus plantarum in submerged bioreactor systems.

Journal of Industrial Microbiology & Biotechnology
Graziela Brusch BrinquesMarco Antônio Záchia Ayub

Abstract

Biomass and lactic acid production by a Lactobacillus plantarum strain isolated from Serrano cheese, a microorganism traditionally used in foods and recognized as a potent probiotic, was optimized. Optimization procedures were carried out in submerged batch bioreactors using cheese whey as the main carbon source. Sequential experimental Plackett-Burman designs followed by central composite design (CCD) were used to assess the influence of temperature, pH, stirring, aeration rate, and concentrations of lactose, peptone, and yeast extract on biomass and lactic acid production. Results showed that temperature, pH, aeration rate, lactose, and peptone were the most influential variables for biomass formation. Under optimized conditions, the CCD for temperature and aeration rate showed that the model predicted maximal biomass production of 14.30 g l(-1) (dw) of L. plantarum. At the central point of the CCD, a biomass of 10.2 g l(-1) (dw), with conversion rates of 0.10 g of cell g(-1) lactose and 1.08 g lactic acid g(-1) lactose (w/w), was obtained. These results provide useful information about the optimal cultivation conditions for growing L. plantarum in batch bioreactors in order to boost biomass to be used as industrial probiotic...Continue Reading

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Citations

Mar 29, 2014·The Journal of Dairy Research·Luisina LavariGabriel Vinderola
Jun 18, 2010·Journal of Industrial Microbiology & Biotechnology·Yang GuoXinlei Wang
Jun 21, 2012·Applied Microbiology and Biotechnology·N BöhmerL Fischer
Aug 16, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Chaline Caren CoghettoMarco Antônio Záchia Ayub
Jul 28, 2016·International Journal of Food Sciences and Nutrition·Chaline Caren CoghettoMarco Antônio Záchia Ayub
Dec 12, 2018·Journal of Dairy Science·Sabrina S SaboRicardo P S Oliveira
Oct 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Przemysław SerugaŁukasz Niedźwiecki
Sep 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hao RenWilfried Vahjen
Apr 14, 2018·BioMed Research International·Fang-Fang JiaXiang-Chen Meng
Jun 9, 2020·3 Biotech·Gabriela Rabaioli RamaClaucia Fernanda Volken de Souza

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