Optimization of silk films as substrate for functional corneal epithelium growth

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Liang JiaDavid L Kaplan


The corneal epithelium is the first cellular barrier to protect the cornea. Thus, functional tissue engineering of the corneal epithelium is a strategy for clinical transplantation. In this study, the optimization of silk films (SFs) as substrates for functional human corneal epithelium growth was investigated with primary human corneal epithelial cells on SFs, poly-D-lysine (PDL) coated SFs, arginine-glycine-aspartic acid (RGD) modified SFs and PDL blended SFs. PDL coated SFs significantly promoted cell adhesion at early phases in comparison to the other study groups, while PDL blended SF significantly promoted cell migration in a "wound healing" model. All film modifications promoted cell proliferation and viability, and a multi-layered epithelium was achieved in 4 weeks of culture. The epithelia formed were tightly apposed and maintained an intact barrier function against rose bengal dye penetration. The results suggested that a differentiated human corneal epithelium can be established with primary corneal epithelial cells on SFs in vitro, by optimizing SF composition with PDL.


May 30, 1997·Science·C S ChenD E Ingber
Dec 11, 1999·Science·M GriffithM A Watsky
Mar 29, 2000·Survey of Ophthalmology·H S Dua, A Azuara-Blanco
Aug 6, 2002·Journal of Biomaterials Science. Polymer Edition·L AucoinH Sheardown
Nov 9, 2002·Biomaterials·Gregory H AltmanDavid L Kaplan
Aug 5, 2003·Biomaterials·Bruce PanilaitisDavid L Kaplan
Aug 19, 2003·Biomaterials·Ulrich HerselHorst Kessler
May 11, 2004·Biomacromolecules·Hyoung-Joon JinDavid L Kaplan
Aug 19, 2004·Journal of Biomedical Materials Research. Part a·Lorenz MeinelDavid L Kaplan
Sep 17, 2004·The New England Journal of Medicine·Kohji NishidaYasuo Tano
Dec 9, 2004·Biomaterials·Ung-Jin KimDavid L Kaplan
Nov 16, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Xianyan WangDavid L Kaplan
Dec 31, 2005·Investigative Ophthalmology & Visual Science·Pablo ArgüesoIlene K Gipson
Jul 4, 2006·Journal of Biomedical Materials Research. Part a·S J LiliensiekC J Murphy
Sep 28, 2006·Investigative Ophthalmology & Visual Science·A HopkinsonHarminder S Dua
Apr 17, 2007·Proteins·Xin ChenFritz Vollrath
Sep 28, 2007·Investigative Ophthalmology & Visual Science·Timothy D BlalockIlene K Gipson
Jan 12, 2008·PloS One·Che J ConnonK M Meek
Mar 26, 2008·Molecular Reproduction and Development·Shengli MiZhongying Dou
Dec 9, 2008·Biomaterials·Brian D LawrenceDavid L Kaplan
Mar 24, 2009·The British Journal of Ophthalmology·Che J ConnonK M Meek
Nov 20, 2009·Macromolecular Bioscience·Qiang LuDavid L Kaplan
Mar 20, 2010·Macromolecular Bioscience·Eun S GilDavid L Kaplan
Jun 25, 2010·The New England Journal of Medicine·Paolo RamaGraziella Pellegrini
Jul 31, 2010·Science·Fiorenzo G Omenetto, David L Kaplan
Feb 22, 2011·Journal of Cataract and Refractive Surgery·Derek W DelMonte, Terry Kim
Jun 5, 2012·Investigative Ophthalmology & Visual Science·Jingbo LiuMark I Rosenblatt
Jun 19, 2012·Acta Biomaterialia·Brian D LawrenceMark I Rosenblatt


Jan 24, 2016·Current Urology Reports·Bryan S SackCarlos R Estrada
Oct 13, 2017·Biomedical Materials·Zhi ChenGordon G Wallace
Sep 22, 2018·Advanced Healthcare Materials·Chris HollandF Philipp Seib
Mar 10, 2017·International Journal of Molecular Sciences·Yu QiKe-Qin Zhang
Sep 29, 2015·Journal of Biomaterials Science. Polymer Edition·John G HardyChristine E Schmidt

Related Concepts

Related Feeds

Barrett Esophagus

Barrett’s esophagus if a serious complication of gastroesophageal reflux disease during which the normal esophageal lining changes to tissue that resembles intestinal lining. Here is the latest research.

Biomaterial Engineering

Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.