PMID: 9542939May 23, 1998Paper

Optimization of specimen-handling procedures for accurate quantitation of levels of human immunodeficiency virus RNA in plasma by reverse transcriptase PCR

Journal of Clinical Microbiology
Ruth DickoverY J Bryson

Abstract

Human immunodeficiency virus type 1 (HIV-1) RNA levels in plasma are currently widely used clinically for prognostication and in monitoring antiretroviral therapy. Accurate and reproducible results are critical for patient management. To determine the effects of specimen collection and handling procedures on quantitative measurement of HIV-1 RNA, we compared anticoagulants and sample processing times. Whole blood was collected from 20 HIV-1-infected patients in EDTA, acid citrate dextrose (ACD), and heparin tubes, aliquoted, and stored at room temperature. Plasma was separated from whole-blood aliquots prepared at < or =1, 3, 6, 24, and 48 h postcollection and then stored at -70 degrees C until use. HIV-1 RNA levels were determined by the AMPLICOR HIV-1 MONITOR assay. Heparinized plasma samples, which were pretreated with heparinase prior to analysis, had the lowest baseline HIV-1 RNA levels. In the first 6 h, HIV-1 RNA levels decreased by 10, 20, and 31% in EDTA, ACD, and heparin tubes, respectively. From 6 to 48 h postcollection, HIV-1 RNA levels decreased in all anticoagulants, albeit at a slower, more consistent rate. Our results indicate that EDTA should be the anticoagulant of choice for plasma HIV-1 RNA measurement by re...Continue Reading

References

Apr 1, 1991·Journal of Clinical Microbiology·M HolodniyT C Merigan
Sep 1, 1990·Journal of Clinical Microbiology·R E DickoverJ R Carlson
Apr 15, 1995·Annals of Internal Medicine·J W MellorsP Gupta
Feb 1, 1994·Journal of Clinical Microbiology·J van BuerenB D Cookson
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·G FangB Weiser

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Citations

Aug 29, 2006·Arquivos brasileiros de endocrinologia e metabologia·Sabrina Mendes CoelhoDenise Pires de Carvalho
Sep 2, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·K AnastosR M Greenblatt
Jun 25, 2005·Clinical Chemistry·Jean-Brice MarteauSophie Visvikis-Siest
May 9, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe BanfiGiuseppe Lippi
Jan 27, 2011·Biomicrofluidics·Sung Hwan ChoYu-Hwa Lo
Sep 27, 2020·Reviews in Medical Virology·Howard Newman, Diana Hardie
Oct 16, 2004·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Ester Cerdeira SabinoLuiz Gastão Rosenfeld
Oct 14, 2017·The Journal of Infectious Diseases·Dolly SinghDeborah Persaud
Apr 19, 2003·Journal of Clinical Microbiology·Daniel R KuritzkesDean L Winslow
Jun 7, 2003·Journal of Clinical Microbiology·M K GhoshG M Aldrovandi
Mar 5, 2003·Antiviral Research·Christian DrostenStephan Günther
May 8, 2009·Journal of Clinical Microbiology·Anne-Marte Bakken KranMona Holberg-Petersen
Mar 30, 2002·Journal of Clinical Microbiology·Marta E GarcíaDomingo Gargallo-Viola

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