Optimization of the Aqueous Extraction of Phenolic Compounds from Olive Leaves

Antioxidants
Chloe D GoldsmithChristopher J Scarlett

Abstract

Olive leaves are an agricultural waste of the olive-oil industry representing up to 10% of the dry weight arriving at olive mills. Disposal of this waste adds additional expense to farmers. Olive leaves have been shown to have a high concentration of phenolic compounds. In an attempt to utilize this waste product for phenolic compounds, we optimized their extraction using water-a "green" extraction solvent that has not yet been investigated for this purpose. Experiments were carried out according to a Box Behnken design, and the best possible combination of temperature, extraction time and sample-to-solvent ratio for the extraction of phenolic compounds with a high antioxidant activity was obtained using RSM; the optimal conditions for the highest yield of phenolic compounds was 90 °C for 70 min at a sample-to-solvent ratio of 1:100 g/mL; however, at 1:60 g/mL, we retained 80% of the total phenolic compounds and maximized antioxidant capacity. Therefore the sample-to-solvent ratio of 1:60 was chosen as optimal and used for further validation. The validation test fell inside the confidence range indicated by the RSM output; hence, the statistical model was trusted. The proposed method is inexpensive, easily up-scaled to industry...Continue Reading

References

Sep 30, 1999·The Journal of Pharmacy and Pharmacology·G BisignanoA Saija
Aug 27, 2002·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·R FabianiG Morozzi
Dec 2, 2004·Nature Reviews. Cancer·Judith S Sebolt-Leopold, Roman Herrera
Sep 2, 2005·Nature·Gary K BeauchampPaul A S Breslin
Feb 21, 2006·Atherosclerosis·Paola BoganiFrancesco Visioli
May 23, 2006·The International Journal of Biochemistry & Cell Biology·Christos TsatsanisAndrew N Margioris
Feb 27, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·María-Isabel Covas
Mar 3, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H K ObiedK Robards
Oct 19, 2007·International Journal of Cancer. Journal International Du Cancer·Yumi Z H-Y HashimChris I R Gill
Dec 19, 2008·Critical Reviews in Food Science and Nutrition·Sara CiceraleRussell S J Keast
Feb 6, 2009·Molecular Nutrition & Food Research·Vlassios GoulasIoannis P Gerothanassis
Jul 8, 2011·Journal of Separation Science·Quan V VuongPaul D Roach
Dec 31, 2011·Journal of Agricultural and Food Chemistry·Amani TaamalliAlberto Fernández-Gutiérrez
Feb 14, 2013·Bioorganic & Medicinal Chemistry·Belnaser A BusnenaKhalid A El Sayed
Mar 26, 2013·Current Medicinal Chemistry·M ScoteceO Gualillo

❮ Previous
Next ❯

Citations

Jul 21, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chloe D GoldsmithChristopher J Scarlett
Apr 25, 2018·Database : the Journal of Biological Databases and Curation·Natalie P BonvinoTom C Karagiannis
Sep 29, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cinzia BenincasaGiovanni Sindona
May 23, 2021·International Journal of Biological Macromolecules·Matheus da Silva CampeloNágila Maria Pontes Silva Ricardo

❮ Previous
Next ❯

Software Mentioned

JMP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.