Optimization of the Biocatalysis for D-DIBOA Synthesis Using a Quick and Sensitive New Spectrophotometric Quantification Method

International Journal of Molecular Sciences
Gema CabreraJorge Bolivar

Abstract

D-DIBOA (4-hydroxy-(2H)-1,4-benzoxazin-3-(4H)-one) is an allelopathic-derived compound with interesting herbicidal, fungicidal, and insecticide properties whose production has been successfully achieved by biocatalysis using a genetically engineered Escherichia coli strain. However, improvement and scaling-up of this process are hampered by the current methodology for D-DIBOA quantification, which is based on high-performance liquid chromatographic (HPLC), a time-consuming technique that requires expensive equipment and the use of environmentally unsafe solvents. In this work, we established and validated a rapid, simple, and sensitive spectrophotometric method for the quantification of the D-DIBOA produced by whole-cell biocatalysis, with limits of detection and quantification of 0.0165 and 0.0501 µmol·mL-1 respectively. This analysis takes place in only a few seconds and can be carried out using 100 µL of the sample in a microtiter plate reader. We performed several whole-cell biocatalysis strategies to optimize the process by monitoring D-DIBOA production every hour to keep control of both precursor and D-DIBOA concentrations in the bioreactor. These experiments allowed increasing the D-DIBOA production from the previously r...Continue Reading

References

Mar 10, 2004·Biotechnology Letters·Julio BerríosGermán Aroca
Apr 12, 2005·Inorganic Chemistry·Natividad GálvezJosé M Domínguez-Vera
Feb 16, 2006·Journal of Agricultural and Food Chemistry·Francisco A MacíasJosé M G Molinillo
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Francisco A MacíasJosé M G Molinillo
Dec 14, 2007·Nature·Benjamin F CravattJohn R Yates
Jun 30, 2015·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Alia Méndez-AlboresEduardo Torres
Jun 15, 2017·Microbial Cell Factories·Baixue Lin, Yong Tao
May 26, 2018·International Journal of Biological Macromolecules·Zahra FathiYusuke Ide

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.