Optimization of the excitation light sheet in selective plane illumination microscopy

Biomedical Optics Express
Liang Gao

Abstract

Selective plane illumination microscopy (SPIM) allows rapid 3D live fluorescence imaging on biological specimens with high 3D spatial resolution, good optical sectioning capability and minimal photobleaching and phototoxic effect. SPIM gains its advantage by confining the excitation light near the detection focal plane, and its performance is determined by the ability to create a thin, large and uniform excitation light sheet. Several methods have been developed to create such an excitation light sheet for SPIM. However, each method has its own strengths and weaknesses, and tradeoffs must be made among different aspects in SPIM imaging. In this work, we present a strategy to select the excitation light sheet among the latest SPIM techniques, and to optimize its geometry based on spatial resolution, field of view, optical sectioning capability, and the sample to be imaged. Besides the light sheets discussed in this work, the proposed strategy is also applicable to estimate the SPIM performance using other excitation light sheets.

References

Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yicong WuHari Shroff
Jun 5, 2012·Nature Methods·Uros KrzicLars Hufnagel
Apr 8, 2014·Nature Methods·Tom VettenburgKishan Dholakia

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Citations

Nov 20, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Soumik SiddhantaIshan Barman
Apr 1, 2017·Nature Methods·Rory M Power, Jan Huisken
Jan 10, 2018·Journal of Biophotonics·Bonifacio Can-UcGustavo A Hirata
Jan 31, 2019·Optics Express·Liang GaoBi-Chang Chen
Feb 6, 2020·Biomedical Optics Express·Elena RemachaFlorian O Fahrbach
Nov 23, 2018·Scientific Reports·Giuseppe Di DomenicoGiuseppe Antonacci

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