Abstract
CGA-N46 is a small antifungal-derived peptide and consists of the 31st-76th amino acids of the N-terminus of human chromogranin A. Polycistronic expression of recombinant CGA-N46 in Bacillus subtilis DB1342 was used to improve its production, but the yield of CGA-N46 was still low. In the present study, response surface methodology (RSM) was used to optimize culture medium composition and growth conditions of the engineered strain B. subtilis DB1342(p-3N46) for the further increase in CGA-N46 yield. The results of two-level factorial experiments indicated that dextrin and tryptone were significant factors affecting CGA-N46 expression. Central composite design (CCD) was used to determine the ideal conditions of each significant factors. From the results of CCD, the optimal medium composition was predicted to be dextrin 16.6 g/L, tryptone 19.2 g/L, KH2PO4·H2O 6 g/L, pH 6.5. And the optimal culture process indicated inoculation of B. subtilis DB1342(p-3N46) seed culture into fresh culture medium at 5 % (v/v), followed by expression of CGA-N46 for 56 hours at 30 °C induced by 2 % (v/v) sucrose after one hour of shaking culture. To test optimal CGA-N46 peptide expression, the yeast growth inhibition assay was employed and it was fou...Continue Reading
References
Apr 1, 1999·Biotechnology and Bioengineering·R YeS L Wong
Apr 23, 2003·Drug Discovery Today·Vida Foubister
Jan 16, 2007·Clinical Microbiology Reviews·M A Pfaller, D J Diekema
Aug 8, 2008·Mikrobiologiia·S Givry, F Duchiro
Mar 28, 2009·Critical Care Medicine·Olivier LeroyUNKNOWN AmarCand Study Group
Jan 22, 2010·Critical Reviews in Microbiology·Michael A Pfaller, Daniel J Diekema
Sep 22, 2011·PloS One·Matteo BassettiClaudio Viscoli
May 11, 2012·Biotechnology and Bioengineering·Katariina M HuttererJennifer Liu
Dec 21, 2012·Science Translational Medicine·Gordon D BrownTheodore C White
Jan 1, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shan HeXiaojun Yan
Mar 28, 2013·World Journal of Microbiology & Biotechnology·Tuğçe DemirE Esin Hameş-Kocabaş
Apr 5, 2013·PloS One·Liliana ScorzoniOscar Zaragoza
Jul 23, 2013·Protein Expression and Purification·Di WuPeilong Yang
Aug 13, 2013·BioMed Research International·Yong-Gang XieYi-Zhen Wang
Sep 3, 2013·Journal of Biotechnology·Lianchun FanChristopher C Frye
Oct 29, 2013·Microbial Cell Factories·Kristof ZarschlerHolger Stephan
Nov 7, 2013·PloS One·Yao NieRong Xiao
Feb 12, 2014·Applied Microbiology and Biotechnology·Zixin PengWeiping Song
Feb 19, 2014·The Journal of Microbiology·Xin ZhaoZhi-jiang Zhou
Mar 13, 2014·SpringerPlus·Xiaomei SuChaofeng Shen
Citations
May 12, 2016·Interdisciplinary Sciences, Computational Life Sciences·Rui-Fang LiChao-Nan Li
Nov 15, 2018·PloS One·Tian Yan YunJiang Hui Xie
Oct 24, 2018·Bioengineering·Albert UhoraningogaBarry J Ryan
Aug 3, 2020·BMC Microbiology·Kaiqi LianYuanchen Zhang