Optimization of the separation of NDA-derivatized methylarginines by capillary and microchip electrophoresis.

Journal of Laboratory Automation
Thomas H LinzS M Lunte

Abstract

The methylated arginines (MAs) monomethylarginine (MMA), asymmetric dimethylarginine (ADMA), and symmetric dimethylarginine (SDMA) have been shown to be independent predictors of cardiovascular disease. This article describes progress regarding the development of an analytical method capable of rapidly analyzing MAs using capillary electrophoresis (CE) and microchip electrophoresis (MCE) with laser-induced fluorescence (LIF) detection. Several parameters including buffer composition and separation voltage were optimized to achieve an ideal separation. The analytes of interest were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) to produce fluorescent 1-cyanobenz[f]isoindole (CBI) derivatives and then subjected to CE analysis. Baseline resolution of SDMA, ADMA, MMA, and arginine was achieved in less than 8 min. The limits of detection for SDMA, ADMA, MMA, and arginine were determined to be 15, 20, 25, and 5 nM, respectively, which are well below the expected plasma concentrations. The CE separation method was then transferred to a glass MCE device with LIF detection. MAs were baseline resolved in 3 min on-chip using a 14 cm separation channel with detection limits of approximately 10 nM for each species. To the best of t...Continue Reading

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Citations

Jan 13, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nathan J ObornySusan M Lunte
Feb 19, 2013·Electrophoresis·Thomas H Linz, Susan M Lunte
Nov 1, 2013·Electrophoresis·Véréna PoinsotFrançois Couderc
Jan 27, 2015·Analytical Methods : Advancing Methods and Applications·Thomas H Linz, Susan M Lunte
Nov 7, 2017·Analytical Methods : Advancing Methods and Applications·Claudia G FrestaSusan M Lunte

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Methods Mentioned

BETA
electrophoresis
chip

Software Mentioned

32
Karat
Labview

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