Optimization of time for neural stem cells transplantation for brain stroke in rats

Stem Cell Investigation
Seyyed Mohyeddin ZiaeeSeyed Mojtaba Hosseini

Abstract

Despite encouraging data in terms of neurological outcome, stem cell based therapy for ischemic stroke in experimental models and human patients is still hampered by multiple as yet un-optimized variables, i.e., time of intervention, that significantly influence the prognosis. The aim of the present study was to delineate the optimum time for neural stem cells (NSCs) transplantation after ischemic stroke. The NSCs were isolated from 14 days embryo rat ganglion eminence and were cultured in NSA medium (neurobasal medium, 2% B27, 1% N2, bFGF 10 ng/mL, EGF 20 ng/mL and 1% pen/strep). The cells were characterized for tri-lineage differentiation by immunocytochemistry for tubulin-III, Olig2 and GFAP expression for neurons, oligodendrocytes and astrocyte respectively. The NSCs at passage 3 were injected intraventricularly in a rodent model of middle-cerebral artery occlusion (MCAO) on stipulated time points of 1 & 12 h, and 1, 3, 5 and 7 days after ischemic stroke. The animals were euthanized on day 28 after their respective treatment. dUTP nick end labeling (TUNEL) assay and Caspase assay showed significantly reduced number of apoptotic cells on day 3 treated animals as compared to the other treatment groups of animals. The neurolog...Continue Reading

Citations

Aug 27, 2018·Journal of Cellular Biochemistry·Seyed Mojtaba HosseiniZahra Abbasi
Jun 14, 2018·Stem Cell Research & Therapy·Austin C BoeseJean-Pyo Lee
Apr 29, 2020·Biotechnology Letters·Hamid Ahmadian-MoghadamMohammad-Reza Zarrindast

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