Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina

The Korean Journal of Parasitology
Pamela TestardiniB Perazzi

Abstract

The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of ...Continue Reading

References

Mar 1, 1995·Sexually Transmitted Diseases·J N Krieger
Mar 1, 1993·Clinical Obstetrics and Gynecology·P Heine, J A McGregor
Oct 24, 1998·Infectious Diseases in Obstetrics and Gynecology·J A SimõesA Faúndes
May 3, 2001·Enfermedades infecciosas y microbiología clínica·S Di BartolomeoR Alberto De Torres
Apr 1, 2004·Sexually Transmitted Infections·H SwygardM S Cohen
Oct 19, 2004·Clinical Microbiology Reviews·J R Schwebke, Donald Burgess
Oct 27, 2005·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Ivana V RadonjicIvana F Kranjcic Zec
Mar 6, 2010·Journal of Clinical Pathology·Evangelia-Theophano PiperakiAthanassios Tsakris
Dec 2, 2010·Revista latino-americana de enfermagem·Danielle Cristina Alves Feitosa GondoCristina Maria Garcia de Lima Parada
Apr 25, 2012·Journal of Global Infectious Diseases·Madhumati J PatilSharada C Metgud
Jun 8, 2012·Journal of the European Academy of Dermatology and Venereology : JEADV·E ShipitsynaMagnus Unemo
May 16, 2014·Sexually Transmitted Diseases·Bronwyn J SilverAlice R Rumbold

Related Concepts

May-Grunwald Giemsa stain
Eosine Yellowish-(YS), Potassium, Sodium Salt
Hospitals, University
Urolene Blue
Microscopy
Statistical Sensitivity
Staining and Labeling
Trichomonas vaginalis
Trichomonas Vaginitis
Pap Smear

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