Optimization on condition of glycyrrhetinic acid liposome by RSM and the research of its immunological activity

International Journal of Biological Macromolecules
Xiaojuan ZhaoZhang Jing

Abstract

The aim of this study is to prepare glycyrrhetinic acid liposome (GAL) and optimize the preparation condition and to investigate further whether liposome could promote the immunological activity of glycyrrhetinic acid (GA). GAL was prepared using a film-dispersion method and the preparation conditions of GAL were optimized with response surface methodology (RSM). Moreover, GAL prepared under the optimal preparation conditions was added into chicken's T and B lymphocytes in vitro. The optimal preparation conditions for GAL by response surface methodology was as follows: ratio 9:1, soybean phospholipid cholesterol (w/w) 2.5:1 and water bath temperature 31 °C. Under these conditions, the experimental encapsulation efficiency of GAL was 83.46 ± 0.55%, which was close with the predicted value. Therefore, the optimized preparation condition is very reliable. The results showed that GAL could significantly promote T and B lymphocytes proliferation singly or synergistically with PHA and LPS and the concentration of immunoglobulins G (IgG) and immunoglobulins M (IgM). These results indicated that liposome could significantly improve the immunological activity of GA and drug action of GA. GAL demonstrates the significant immunological ac...Continue Reading

References

Mar 1, 1990·Immunology Today·G Gregoriadis
Jul 10, 1999·Toxicon : Official Journal of the International Society on Toxinology·G LinT Y Cheng
Mar 13, 2003·Developmental and Comparative Immunology·Takuya IrieHiroshi Kodama
Feb 29, 2008·International Journal of Pharmaceutics·Yang LuGuangji Wang
Apr 14, 2009·European Journal of Pharmacology·Panneerselvam KalaiarasiKodukkur Viswanathan Pugalendi
Sep 4, 2010·International Journal of Pharmaceutics·Qin TianZhi Yuan

❮ Previous
Next ❯

Citations

Nov 26, 2013·International Journal of Pharmaceutics·Yun YuDeyun Wang
Mar 24, 2015·Journal of Liposome Research·Yan Ge, Mingqiao Ge
Jul 12, 2014·International Journal of Pharmaceutics·Huijuan WangSong Du
Jun 30, 2015·International Journal of Biological Macromolecules·Yun ChenYuanliang Hu
Aug 19, 2015·International Journal of Biological Macromolecules·Li-Hong TanWei-Guo Cao
Jun 5, 2013·International Journal of Biological Macromolecules·Yun YuHuarong Pan
Jun 28, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Bin ZhengJing Xie
Nov 28, 2013·Carbohydrate Polymers·Xuejing JiaMing Yuan
Jun 9, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Wenping WangHong Wang
Feb 16, 2019·Drug Development and Industrial Pharmacy·Sayan BiswasPritorthi Bhattacharjee
Aug 1, 2015·Journal of Microencapsulation·Sha-Sha LiQiang Liu
Oct 17, 2020·Pharmaceutical Development and Technology·Sayan BiswasThidarat Duangyod

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.