Optimized 5-hour multiplex PCR test for the detection of tinea unguium: performance in a routine PCR laboratory

Medical Mycology
Anna Brillowska-DabrowskaMaiken Cavling Arendrup

Abstract

We recently reported the development of a 5-hour multiplex PCR test for the detection of tinea unguium and the optimization of this test by the inclusion of an inhibition control. Here we report the performance of this procedure as used in a routine clinical laboratory as compared to conventional microscopy and culture-based techniques performed in a mycology reference laboratory. We found in processing 109 samples that 22 (20.2%) yielded fungi in culture while the suspected etiologic agents were noted microscopically in 15 (13.8%) that were negative in culture. Fungi were detected by PCR in 37 (33.9%) samples, of which only three were positive in culture. Since the majority of PCR positive but culture negative samples were positive in microscopic examinations, the increased sensitivity was not due to contamination. PCR inhibitors were present in 5% of the samples, but this was overcome by re-running the samples with a 50% reduction of sample DNA. In conclusion, the PCR test performance in the routine setting was excellent and provided a markedly reduced time to diagnosis with a higher sensitivity.

References

Apr 1, 1995·Clinical Microbiology Reviews·I Weitzman, R C Summerbell
Jan 9, 2003·Journal of Clinical Microbiology·Jørgen Skov JensenBirthe Dohn
Apr 21, 2004·Journal of the American Academy of Dermatology·K Wade FosterBoni E Elewski
Apr 7, 2006·Journal of Clinical Microbiology·V KardjevaY Gräser
Feb 3, 2007·Journal of Clinical Microbiology·Anna Brillowska-DabrowskaMaiken Cavling Arendrup
Nov 23, 2007·Indian Journal of Dermatology, Venereology and Leprology·Mudita GuptaGita Ram Tegta

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Citations

Apr 26, 2012·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·J Brasch
Dec 16, 2011·Journal of Clinical Microbiology·Julie VerrierMichel Monod
Dec 3, 2014·Journal de mycologie médicale·D PetinataudM Machouart
Jan 18, 2012·Current Opinion in Infectious Diseases·Rasmus H Jensen, Maiken C Arendrup
Feb 22, 2013·The Australasian Journal of Dermatology·Nisha Suyien ChandranChew-Swee Seow
Nov 25, 2014·Journal of Medical Microbiology·Anastasia SpiliopoulouMyrto Christofidou
Mar 5, 2014·Journal of Microbiological Methods·Mariusz WorekMaciej Wnuk
Oct 21, 2016·Journal of the European Academy of Dermatology and Venereology : JEADV·N KobylakA Brillowska-Dąbrowska
Jul 30, 2016·Mycoses·Eleonora DubljaninIvana Čolović Čalovski
Apr 11, 2018·Antimicrobial Agents and Chemotherapy·Milena KordalewskaDavid S Perlin
Sep 19, 2019·Journal of Medical Microbiology·Seok Hwee KooSu Ping Regina Lim
Feb 1, 2020·Journal of Basic Microbiology·Jubeda BegumKapil Dev
Feb 13, 2020·Journal of Applied Microbiology·S GnatA Nowakiewicz
May 15, 2020·Transboundary and Emerging Diseases·Sebastian GnatMariusz Dyląg
Oct 9, 2019·Antimicrobial Agents and Chemotherapy·Milena KordalewskaDavid S Perlin
Sep 5, 2019·Frontiers in Microbiology·Milena Kordalewska, David S Perlin
Aug 3, 2016·Mycopathologia·Julie Verrier, Michel Monod
Mar 1, 2019·Journal of Fungi·Michel Monod, Bruno Méhul
Jan 12, 2021·International Journal of Veterinary Science and Medicine·Hassan Aboul-EllaHeidy Abo-Elyazeed
May 26, 2017·Journal of Clinical Microbiology·Milena KordalewskaDavid S Perlin

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