Optimized Analytical Procedures for the Untargeted Metabolomic Profiling of Human Urine and Plasma by Combining Hydrophilic Interaction (HILIC) and Reverse-Phase Liquid Chromatography (RPLC)-Mass Spectrometry.

Molecular & Cellular Proteomics : MCP
Kévin ContrepoisMichael Snyder

Abstract

Profiling of body fluids is crucial for monitoring and discovering metabolic markers of health and disease and for providing insights into human physiology. Since human urine and plasma each contain an extreme diversity of metabolites, a single liquid chromatographic system when coupled to mass spectrometry (MS) is not sufficient to achieve reasonable metabolome coverage. Hydrophilic interaction liquid chromatography (HILIC) offers complementary information to reverse-phase liquid chromatography (RPLC) by retaining polar metabolites. With the objective of finding the optimal combined chromatographic solution to profile urine and plasma, we systematically investigated the performance of five HILIC columns with different chemistries operated at three different pH (acidic, neutral, basic) and five C18-silica RPLC columns. The zwitterionic column ZIC-HILIC operated at neutral pH provided optimal performance on a large set of hydrophilic metabolites. The RPLC columns Hypersil GOLD and Zorbax SB aq were proven to be best suited for the metabolic profiling of urine and plasma, respectively. Importantly, the optimized HILIC-MS method showed excellent intrabatch peak area reproducibility (CV < 12%) and good long-term interbatch (40 days...Continue Reading

References

Sep 27, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Helena IdborgSven P Jacobsson
Sep 15, 2006·Journal of Separation Science·Petrus Hemström, Knut Irgum
Nov 2, 2007·Analytical Chemistry·Simon CubbonJane Thomas-Oates
Dec 19, 2007·Analytical Chemistry·Anders NordströmGary Siuzdak
Jan 11, 2008·Annual Review of Pharmacology and Toxicology·Rima Kaddurah-DaoukRichard M Weinshilboum
Nov 15, 2008·Molecular & Cellular Proteomics : MCP·Kyoungmi KimRobert H Weiss
Feb 25, 2009·Analytical Chemistry·Jörg Martin BüscherNicola Zamboni
Jun 27, 2009·Mass Spectrometry Reviews·Simon CubbonJane Thomas-Oates
Feb 6, 2010·Journal of Separation Science·Sylvia Van DorpeBart De Spiegeleer
Feb 27, 2010·Journal of Separation Science·Konstantina SpagouGeorgios Theodoridis
Mar 27, 2010·Journal of Chromatography. a·Raluca-Ioana ChiritaClaire Elfakir
May 8, 2010·Nature Protocols·Elizabeth J WantJeremy K Nicholson
Dec 15, 2010·Analytical Chemistry·Konstantina SpagouElizabeth J Want
Feb 19, 2011·Analytical Chemistry·Oscar YanesGary Siuzdak
Mar 2, 2011·PloS One·Nikolaos PsychogiosDavid S Wishart
Jul 9, 2011·Journal of Chromatography. a·Yong Guo, Sheetal Gaiki
Sep 13, 2011·Journal of Proteome Research·Sara TulipaniCristina Andrés-Lacueva
Mar 23, 2012·Nature Reviews. Molecular Cell Biology·Gary J PattiGary Siuzdak
Dec 12, 2012·Nature Protocols·Elizabeth J WantJeremy K Nicholson
Mar 19, 2013·Molecular Nutrition & Food Research·Ciara MorrisLorraine Brennan
Mar 20, 2013·Chemical Reviews·Naomi L Kuehnbaum, Philip Britz-McKibbin
Jul 3, 2013·Omics : a Journal of Integrative Biology·Bedilu Alamirie EjiguMaya Berg
Jul 31, 2013·Clinical Chemistry·Mary K TownsendBrian M Wolpin
Sep 12, 2013·PloS One·Souhaila BouatraDavid S Wishart
Jan 22, 2014·Journal of Proteome Research·Maj-Britt S AndersenLars O Dragsted
May 13, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Samia BoudahChristophe Junot
Oct 7, 2014·Mass Spectrometry Reviews·Dao-Quan TangChoon Nam Ong

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Citations

Mar 5, 2016·Nature Reviews. Rheumatology·Monica GumaGary S Firestein
Apr 30, 2016·International Journal of Molecular Sciences·Ina Aretz, David Meierhofer
Oct 6, 2016·Cell Reports·Indumathi ChennamsettyJoshua W Knowles
Oct 25, 2016·Current Opinion in Biotechnology·Jennifer Haggarty, Karl Ev Burgess
Nov 5, 2016·Analytical Chemistry·Lukáš NajdekrTomáš Adam
Dec 20, 2016·Journal of Proteome Research·Patrick LeutholdMathias Haag
Oct 30, 2016·Journal of Separation Science·Isabelle Kohler, Martin Giera
Nov 1, 2017·Critical Reviews in Clinical Laboratory Sciences·Diana Navas-CarrilloEsteban Orenes-Piñero
Sep 26, 2017·Metabolomics : Official Journal of the Metabolomic Society·Kefeng LiRobert K Naviaux
Apr 28, 2018·Circulation Research·Ryan A KelloggMichael P Snyder
Feb 28, 2018·Mass Spectrometry Reviews·Liang CuiYie Hou Lee
Nov 21, 2018·Omics : a Journal of Integrative Biology·Qingwei MaWei Wang
Nov 28, 2018·Nature Immunology·Paras S MinhasKatrin I Andreasson
Nov 21, 2019·Aging Cell·Bryan J BunningSandra Andorf
Aug 15, 2019·Rapid Communications in Mass Spectrometry : RCM·Koshi NagaiYoshihisa Tomioka
Aug 9, 2016·Journal of Occupational and Environmental Medicine·Ken H LiuDean P Jones
Jun 17, 2020·Expert Review of Neurotherapeutics·Roberta BonomoDebra J Skene
May 1, 2018·Metabolomics : Official Journal of the Metabolomic Society·Lauren PetrickStephen Rappaport
Sep 10, 2019·Metallomics : Integrated Biometal Science·Luis GalvezGunda Koellensperger
Mar 21, 2018·Frontiers in Aging Neuroscience·Daniel StoesselWalter Maetzler
Dec 12, 2018·Scientific Reports·Kévin ContrepoisMichael Snyder
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Adam M KingRobert Trengove
Dec 20, 2017·Analytical and Bioanalytical Chemistry·Fuad J NaserGary J Patti
May 31, 2019·Nature·Wenyu ZhouMichael Snyder
May 10, 2019·Nature Medicine·Sophia Miryam Schüssler-Fiorenza RoseMichael P Snyder
May 30, 2020·Cell·Kévin ContrepoisMichael P Snyder

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Methods Mentioned

BETA
nuclear magnetic resonance
metabolomic profiling

Software Mentioned

R
CAMERA
MassHunter Qualitative Analysis
Zorbax
Hypersil GOLD
XCMS

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