Optimized Bone Regeneration in Calvarial Bone Defect Based on Biodegradation-Tailoring Dual-shell Biphasic Bioactive Ceramic Microspheres

Scientific Reports
Antian XuZhongru Gou

Abstract

Bioceramic particulates capable of filling bone defects have gained considerable interest over the last decade. Herein, dual-shell bioceramic microspheres (CaP@CaSi@CaP, CaSi@CaP@CaSi) with adjustable beta-tricalcium phosphate (CaP) and beta-calcium silicate (CaSi) distribution were fabricated using a co-concentric capillary system enabling bone repair via a tailorable biodegradation process. The in vitro results showed the optimal concentration (1/16 of 200 mg/ml) of extracts of dual-shell microspheres could promote bone marrow mesenchymal cell (BMSC) proliferation and enhance the level of ALP activity and Alizarin Red staining. The in vivo bone repair and microsphere biodegradation in calvarial bone defects were compared using micro-computed tomography and histological evaluations. The results indicated the pure CaP microspheres were minimally resorbed at 18 weeks post-operatively and new bone tissue was limited; however, the dual-shell microspheres were appreciably biodegraded with time in accordance with the priority from CaSi to CaP in specific layers. The CaSi@CaP@CaSi group showed a significantly higher ability to promote bone regeneration than the CaP@CaSi@CaP group. This study indicates that the biphasic microspheres w...Continue Reading

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Citations

Feb 20, 2020·EFORT Open Reviews·Hagen SchmalUNKNOWN Danish Orthopaedic Trauma Society
Jun 17, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Suelen PainiGerson Francisco Assis
Jul 29, 2021·Biomedical Physics & Engineering Express·George Gonçalves Dos SantosFabiana Paim Rosa

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Methods Mentioned

BETA
scanning electric microscopy
Protein Assay

Software Mentioned

GraphPad Prism
μCT
VGStudio Max

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