Jul 9, 2014

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

Proceedings of the National Academy of Sciences of the United States of America
Fillip PortSimon L Bullock

Abstract

The type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system has emerged recently as a powerful method to manipulate the genomes of various organisms. Here, we report a toolbox for high-efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) expression plasmids. Systematic evaluation reveals Cas9 lines with ubiquitous or germ-line-restricted patterns of activity. We also demonstrate differential activity of the same gRNA expressed from different U6 snRNA promoters, with the previously untested U6:3 promoter giving the most potent effect. An appropriate combination of Cas9 and gRNA allows targeting of essential and nonessential genes with transmission rates ranging from 25-100%. We also demonstrate that our optimized CRISPR/Cas tools can be used for offset nicking-based mutagenesis. Furthermore, in combination with oligonucleotide or long double-stranded donor templates, our reagents allow precise genome editing by homology-directed repair with rates that make selection markers unnecessary. Last, we demonstrate a novel application of CRISPR/Cas-mediated technology in revealing loss-of-function phenotypes in somati...Continue Reading

  • References35
  • Citations184

Mentioned in this Paper

Genes, Insect
Shuttle Vectors
Genome
CRISPR-Cas Systems
Base Excision Repair
Genetic Screening (Procedure)
Promoter
Drosophila
Recombinant Transgenes
Gametes

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