Optimized Cryopreservation and Banking of Human Bone-Marrow Fragments and Stem Cells

Biopreservation and Biobanking
Gianluca CarnevaleAnto de Pol

Abstract

Adult mesenchymal stem cells are a promising source for cell therapies and tissue engineering applications. Current procedures for banking of human bone-marrow mesenchymal stem cells (hBM-MSCs) require cell isolation and expansion, and thus the use of large amounts of animal sera. However, animal-derived culture supplements have the potential to trigger infections and severe immune reactions. The aim of this study was to investigate an optimized method for cryopreservation of human bone-marrow fragments for application in cell banking procedures where stem-cell expansion and use are not immediately needed. Whole trabecular fragments enclosing the bone marrow were stored in liquid nitrogen for 1 year in a cryoprotective solution containing a low concentration of dimethyl sulfoxide and a high concentration of human serum (HuS). After thawing, the isolation, colony-forming-unit ability, proliferation, morphology, stemness-related marker expression, cell senescence, apoptosis, and multi-lineage differentiation potential of hBM-MSCs were tested in media containing HuS compared with hBM-MSCs isolated from fresh fragments. Human BM-MSCs isolated from cryopreserved fragments expressed MSC markers until later passages, had a good prolif...Continue Reading

References

Feb 1, 1997·Journal of Cellular Biochemistry·N JaiswalS P Bruder
Apr 2, 1999·Science·M F PittengerD R Marshak
May 13, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J E DennisA I Caplan
Jun 10, 2000·British Journal of Haematology·A EricesJ J Minguell
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Aug 6, 2003·Blood·Pieternella S In 't AnkerHumphrey H H Kanhai
May 4, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jeffrey L SpeesDarwin J Prockop
Oct 26, 2005·Journal of Cellular Physiology·Megan A ForemanStephen J Publicover
Dec 13, 2007·Stem Cell Reviews·Wolfgang Wagner, Anthony D Ho
Nov 16, 2010·Annual Review of Pathology·Nora G Singer, Arnold I Caplan
May 7, 2013·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Gianluca CarnevaleAnto De Pol
May 3, 2014·Cell and Tissue Research·Tullia MaraldiAnto De Pol

❮ Previous
Next ❯

Citations

Sep 17, 2018·Stem Cell Research & Therapy·Irina ArutyunyanGennady Sukhikh
Nov 13, 2018·Bulletin of Experimental Biology and Medicine·I V ArutyunyanТ Kh Fatkhudinov
Dec 11, 2020·Journal of the American Chemical Society·Xing LiShengyi Dong

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence-activated cell sorting
FACS

Software Mentioned

Adobe Photoshop
ImageJ
GraphPad Prism
MacOS
FlowJo
NIS

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis