PMID: 3772111Nov 6, 1986Paper

Optimized density gradient separation of leukocyte fractions from whole blood by adjustment of osmolarity

Journal of Immunological Methods
M Braide, L M Bjursten

Abstract

Some of the compounds used for density gradient separation of blood cells have high osmolarities at the concentrations needed to create the required specific densities. Several mixed media use a combination of hyperosmolar shrinkage and red cell aggregation to improve cell separation. Due to the characteristics of Percoll density gradient medium the density and osmolarity of the gradient can be controlled separately. In the present study, Percoll gradients were used to determine the buoyant densities of different human blood cells at the osmolarities 300 mosM, 350 mosM and 400 mosM. Cell volumes were measured at the same osmolarities using a Coulter counter with channelyzer. As expected, the cell buoyant densities increased and the cell volumes decreased at the higher osmolarities used. There were, however, quantitative differences between the cells with respect to the effects of an increased osmolarity, making a 350 mosM density gradient the most effective in separating mononuclear leukocytes from polymorphonuclear leukocytes. A 400 mosM gradient offered the best possibilities to separate red blood cells from polymorphonuclear leukocytes. A one-step centrifugation procedure, based on these principles, is presented. This proced...Continue Reading

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Citations

Oct 23, 1987·Journal of Immunological Methods·R L RobertsE R Stiehm
Sep 2, 1998·Journal of Biomaterials Science. Polymer Edition·H Nygren, M Broberg
Oct 3, 2006·Journal of Biomedical Materials Research. Part a·Ramiro ContrerasJohn A Frangos
Dec 14, 2005·Journal of Biomedical Materials Research. Part a·Herman SahlinJohn A Frangos
Nov 1, 1996·The Journal of Laboratory and Clinical Medicine·C KarlssonM Braide
Oct 1, 1988·European Journal of Cancer & Clinical Oncology·G BlomqvistG Skolnik
Nov 2, 2016·Preparative Biochemistry & Biotechnology·Narges RahmanianAmir-Hassan Zarnani
Apr 1, 1991·Biological Chemistry Hoppe-Seyler·L RoxvallM Heideman
Jul 9, 1999·Journal of Biomedical Materials Research·H NygrenI Lundström
Feb 1, 1988·Biological Reviews of the Cambridge Philosophical Society·D P Green
Aug 1, 1991·British Journal of Haematology·P MoreauJ L Harousseau
Jun 2, 2001·Alternatives to Laboratory Animals : ATLA·H Sahlin, H Nygren
May 1, 2000·Alternatives to Laboratory Animals : ATLA·H NygrenN Yahyapour
Jan 22, 1998·The American Journal of Physiology·B P HelmkeG W Schmid-Schönbein

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