Optimized lysis buffer reagents for solubilization and preservation of proteins from cells and tissues

Drug Delivery and Translational Research
Byeong Hee HwangSamir Mitragotri

Abstract

Reagents that facilitate solubilization of cells and tissues while preserving the biological activity of their constituents play a major role in various applications including drug delivery. Such reagents are necessary for the accurate determination of cellular and tissue concentrations of proteins, peptides, and nucleic acids, and to measure therapeutic efficacy of drug delivery technologies. Surfactant-based reagents are commonly used for this purpose; however, their utility is marred either by limited ability to solubilize or tendency to denature the proteins during solubilization. Here, we report on the screening and identification of combinations of nonionic and zwitterionic surfactants that possess excellent ability to solubilize mechanically strong and elastic tissues such as skin, while preserving its protein constituents. The leading combination, comprising an equi-mass mixture of 3-(N,N-dimethyl myristyl ammonio) propanesulfonate (TPS, CAS number:14933-09-6) and polyoxyethylene(10) cetyl ether (Brij® C10, CAS number: 9004-95-9) with a total surfactant concentration 0.5 % w/v, solubilized keratinocytes and preserved the activity of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) enzyme in its extracts at room temperat...Continue Reading

References

Oct 19, 1999·Molecular and Cellular Biology·B FutcherJ I Garrels
Jun 22, 2000·Journal of Pharmaceutical Sciences·S MitragotriR Langer
Jan 10, 2002·Journal of Pharmaceutical Sciences·Ahmet TezelSamir Mitragotri
Apr 30, 2003·Journal of Structural Biology·David HosfieldRaymond C Stevens
Dec 1, 2005·Journal of the American Chemical Society·Karina L HerediaHeather D Maynard
Jan 19, 2006·Therapeutic Drug Monitoring·Michael OellerichVictor W Armstrong
Aug 3, 2007·Clinical Pharmacology and Therapeutics·D K ArrellA Terzic
Apr 23, 2008·Journal of the Royal Society, Interface·Robert B Brown, Julie Audet
Feb 17, 2009·Nature Reviews. Microbiology·Nathan C VerBerkmoesJillian F Banfield
Nov 20, 1939·The Journal of General Physiology·M L Anson
Dec 17, 2009·Journal of Biomedicine & Biotechnology·Siun Chee Tan, Beow Chin Yiap
Apr 3, 2010·Molecular BioSystems·Benjamin D CosgroveDouglas A Lauffenburger
Jul 21, 2010·Angewandte Chemie·Liesbeth J De CockMaria N Antipina
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sumit PaliwalSamir Mitragotri
Dec 3, 2010·The Journal of Biological Chemistry·Terence A AgborCormac T Taylor
Mar 25, 2011·Expert Opinion on Drug Delivery·Katsuhiko ArigaJonathan P Hill
Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shoko KurodaYoshiko Iwai
Nov 22, 2011·Nanotechnology·Cristiana S O PauloLino S Ferreira
Nov 20, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sumit PaliwalSamir Mitragotri
Oct 9, 2009·Advanced Functional Materials·Seung-Woo ChoDaniel G Anderson
Oct 1, 2012·Drug Delivery and Translational Research·Byeong Hee HwangSamir Mitragotri

❮ Previous
Next ❯

Citations

Aug 25, 2018·Pancreas·Hirotake KomatsuYoko Mullen
Jun 20, 2020·The Journal of Investigative Dermatology·Elnara MuradovaKenneth Y Tsai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.