Optimized preparation of pDNA/poly(ethylene imine) polyplexes using a microfluidic system

Lab on a Chip
Heiko DebusThomas Kissel

Abstract

Poly(ethylene imine) (PEI) is an established non-viral vector system for the delivery of various nucleic acids in gene therapy applications. Polyelectrolyte complexes between both compounds, so called polyplexes, are formed by electrostatic interactions of oppositely charged macromolecules and are thought to facilitate uptake into cells. Such complexes form spontaneously and on lab scale they are usually prepared by mixing solutions through pipetting. Hence, an optimized preparation procedure allowing the scale-up of well-defined polyplexes would be of general interest. We developed a new method for microfluidic polyplex preparation on a chip. The mixing behaviour within the microfluidic channels was evaluated. Polyplexes with PEI and plasmid DNA were prepared using this method, in comparison to the standard pipetting procedure. Sizes and polydispersity indices of these complexes were examined. The influence of various parameters on the polyplex characteristics and the suitability of this production procedure for other PEI-based complexes were also evaluated. It was shown that polyplexes could easily be prepared by microfluidics. The ratio of PEI to DNA was most important for the formation of small polyplexes, whereas other par...Continue Reading

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Dec 18, 2013·ACS Nano·Mengqian LuTony Jun Huang
Jan 18, 2013·Therapeutic Delivery·Florian SchlenkDagmar Fischer
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Nov 7, 2021·Advanced Healthcare Materials·Evan Kurt, Tatiana Segura

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