Optimized production of extracellular alkaline protease from Aspergillus tamarii with natural by-products in a batch stirred tank bioreactor.

Preparative Biochemistry & Biotechnology
Nagarajan ArumugamSurianarayanan Mahadevan

Abstract

Proteolytic enzymes are one of the significant commercially manufactured enzymes. The manufacture of extracellular alkaline protease by Aspergillus tamarii MTCC5152 was explored using several agricultural by-products as substrates viz., cottonseed meal, wheat bran, skimmed milk and soya flour in submerged fermentation, were found to be efficient for enzyme production and commercially significant. Response surface methodology (RSM) is a statistics-based experimental design, sourced to explore the impact of physical parameters on the manufacture of protease from A. tamarii in a batch stirred tank bioreactor (STBR). The four substantial variables (pH, temperature, inoculum size, and agitation) were carefully chosen for optimization analyses and the statistical pattern was created using a central composite design and the quadratic model has been developed. The optimum conditions for protease production (1.51 U mL-1) where: pH 6.4, temperature 27 °C, inoculum size 2.6%, and agitation 327 rpm. The analysis revealed that the anticipated values were in accord with trial data with a correlation coefficient of 0.969.

References

Oct 6, 2005·Bioresource Technology·V J MehtaS P Singh
Jan 25, 2007·Journal of Industrial Microbiology & Biotechnology·Dayanandan AnandanRobert L Dudley
Sep 15, 2010·Indian Journal of Pharmaceutical Sciences·P KamathP Vasantha Raj
Nov 25, 2011·Applied Microbiology and Biotechnology·Balaji DhandapaniAsit Baran Mandal
Dec 12, 2012·Journal of Microbiology and Biotechnology·Biswanath BhuniaApurba Dey
Aug 12, 2014·Enzyme Research·Nilesh P Nirmal, R Seeta Laxman
Jun 21, 2018·International Journal of Biological Macromolecules·Parthiban SujithaChittibabu Shanthi
Jun 1, 2017·Journal, Genetic Engineering & Biotechnology·Kanupriya Miglani SharmaAshwani Kumar

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Methods Mentioned

BETA
protease assay

Software Mentioned

iControl RC1e
Minitab
Matlab

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