Optimized proteomic analysis on gels of cell-cell adhering junctional membrane proteins

Biochemistry
Yuji YamazakiSachiko Tsukita

Abstract

A high level of structural organization of functional membrane domains in very narrow regions of a plasma membrane is crucial for the functions of plasma membranes and various other cellular functions. Conventional proteomic analyses are based on total soluble cellular proteins. Thus, because of insolubility problems, they have major drawbacks for use in analyses of low-abundance proteins enriched in very limited and specific areas of cells, as well as in analyses of the membrane proteins in two-dimensional gels. We optimized proteomic analyses of cell-cell adhering junctional membrane proteins on gels. First, we increased the purity of cell-cell junctions, which are very limited and specific areas for cell-cell adhesion, from hepatic bile canaliculi. We then enriched junctional membrane proteins via a guanidine treatment; these became selectively detectable on two- dimensionally electrophoresed gels after treatment with an extremely high concentration of NP-40. The framework of major junctional integral membrane proteins was shown on gels. These included six novel junctional membrane proteins of type I, type II, and tetraspanin, which were identified by mass spectrometry and by a database sequence homology search, as well as 1...Continue Reading

References

Jan 1, 1989·The Journal of Cell Biology·S Tsukita, S Tsukita
Dec 1, 1993·The Journal of Cell Biology·S TsukitaS Tsukita
Feb 5, 1998·Journal of Mass Spectrometry : JMS·J R Yates
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·K J Green, C A Gaudry
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·S TsukitaM Itoh
Jan 5, 2002·The Journal of Cell Biology·M E Hemler
Feb 26, 2003·Cell·Mirna Perez-MorenoElaine Fuchs
Mar 1, 2003·Nature Biotechnology·Christine C Wu, John R Yates
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Leonard J FosterMatthias Mann
Sep 21, 2004·Proteomics·Holly D CoughenourCharles M Thompson
Mar 31, 2005·Cell·Rebecca A IhrieLaura D Attardi
Jul 28, 2005·Journal of Neuroscience Research·Greg R PhillipsDavid R Colman
Aug 16, 2005·Current Opinion in Cell Biology·Karl MatterMaria S Balda
Oct 19, 2005·Nature Reviews. Molecular Cell Biology·John R YatesJohn J M Bergeron
Jul 18, 2006·Cellular and Molecular Life Sciences : CMLS·L-J ZhangS Liang
Nov 7, 2006·Developmental Cell·Mirna Perez-Moreno, Elaine Fuchs
Nov 23, 2006·The Journal of Cell Biology·Georg H H BornerMargaret S Robinson

❮ Previous
Next ❯

Citations

Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·James M Anderson, Christina M Van Itallie
Nov 23, 2011·The Journal of Cell Biology·Beate K StraubWerner W Franke
Apr 13, 2011·The Journal of Cell Biology·Tomoki YanoSachiko Tsukita
Feb 1, 2011·FEBS Letters·Katsuhiko MinetaSachiko Tsukita
Dec 19, 2015·Journal of Proteome Research·Fei LiuMark S Baker
May 29, 2014·Tissue Barriers·Hui YouStelios T Andreadis
Aug 31, 2014·Seminars in Cell & Developmental Biology·Christina M Van Itallie, James M Anderson
Jun 8, 2017·Frontiers in Immunology·Qinghui MuXin M Luo
Apr 5, 2013·The Journal of Biological Chemistry·Christina M Van ItallieJames Melvin Anderson
Aug 23, 2020·Journal of Clinical Medicine·Agostino Di CiaulaPiero Portincasa
Jan 19, 2020·Cancers·Marek MazurekCezary Grochowski
Sep 24, 2019·World Journal of Gastroenterology : WJG·Alberto NicolettiAntonio Grieco
Feb 23, 2021·Advanced Drug Delivery Reviews·Joël BrunnerGerrit Borchard
Mar 22, 2021·The Journal of Biological Chemistry·Fei LiRobert M Stroud

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

American Journal of Physiology. Cell Physiology
Eveline E Schneeberger, Robert D Lynch
Cold Spring Harbor Perspectives in Biology
Wenxiang Meng, Masatoshi Takeichi
© 2022 Meta ULC. All rights reserved