Optimized Protocol for OnGuard2 Software in Studying Guard Cell Membrane Transport and Stomatal Physiology

Frontiers in Plant Science
Sehar ShafaqueYizhou Wang

Abstract

Stomata are key innovation in plants that drives the global carbon and water cycle. In the past few decades, many stomatal models have been developed for studying gas exchange, photosynthesis, and transpirational characteristics of plants, but they provide limited information on stomatal mechanisms at the molecular and cellular levels. Quantitative mathematical modeling offers an effective in silico approach to explore the link between microscopic transporter functioning and the macroscopic stomatal characteristics. As a first step, a dynamic system model based on the guard cell membrane transport system was developed and encoded in the OnGuard software. This software has already generated a wealth of testable predictions and outcomes sufficient to guide phenotypic and mutational studies. It has a user-friendly interface, which can be easily accessed by researchers to manipulate the key elements and parameters in the system for guard cell simulation in plants. To promote the adoption of this OnGuard application, here we outline a standard protocol that will enable users with experience in basic plant physiology, cell biology, and membrane transport to advance quickly in learning to use it.

References

Oct 14, 2000·Annual Review of Cell and Developmental Biology·M R Blatt
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Julian I SchroederDavid Waner
Dec 19, 2001·Cell·A M Hetherington
Aug 22, 2003·Nature·Alistair M Hetherington, F Ian Woodward
Jul 22, 2008·The Biochemical Journal·Lionel VerdoucqChristophe Maurel
Jun 16, 2010·Plant, Cell & Environment·Gaëlle DamourLaurent Urban
Jul 2, 2010·Plant Molecular Biology·Jorge BellatiGabriela Amodeo
Jun 5, 2013·Plant, Cell & Environment·Thomas N Buckley, Keith A Mott
Dec 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yin WangToshinori Kinoshita
Mar 1, 2014·Plant Physiology·Tracy Lawson, Michael R Blatt
Dec 9, 2014·Plant Signaling & Behavior·Tong ZhangAlice C Harmon
Sep 4, 2015·Physiological Reviews·Christophe MaurelLionel Verdoucq
Jan 8, 2017·Plant Physiology·Thomas N Buckley
Apr 7, 2017·Plant Physiology·Cornelia Eisenach, Alexis De Angeli
Apr 15, 2017·Plant Physiology·Mareike Jezek, Michael R Blatt
Jun 7, 2017·Plant Physiology·Michael R BlattKeiko U Torii
Feb 25, 2019·Trends in Plant Science·Frances C SussmilchM Rob G Roelfsema
Apr 30, 2019·Plant, Cell & Environment·Mareike JezekVirgilio L Lew

❮ Previous
Next ❯

Software Mentioned

Windows
Origin
SigmaPlot
CM
Bootcamp
OnGuard
Win64
Windows10
Win32
HoTsig

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.