Optimized recombinant dense bodies of human cytomegalovirus efficiently prime virus specific lymphocytes and neutralizing antibodies without the addition of adjuvant

Vaccine
Sabine BeckeSabine Reyda

Abstract

Control of human cytomegalovirus (HCMV) infection correlates with the reconstitution of antiviral T lymphocytes in haematopoietic stem cell transplant recipients. A vaccine to foster this reconstitution and to ameliorate the severe consequences of HCMV reactivation is yet unavailable. This work focused on providing a rationale for the amendment of the yields and the antigenic composition of a vaccine, based on subviral dense bodies (DB) of HCMV. Modified DB were generated that contained the HLA-A2 presented IE1 model peptide TMYGGISLL, integrated at different positions in the major DB protein pp65. Insertion at position W175 of pp65 allowed efficient formation of recDB in the cytoplasm of infected cells and resulted in considerable yields of these particles. Even in the absence of adjuvant, these particles proved to be highly immunogenic with respect to CD8 and CD4 T cell and neutralizing antibody responses.

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Citations

Jun 12, 2013·Expert Review of Vaccines·Vijayendra DasariRajiv Khanna
Mar 21, 2015·Medical Microbiology and Immunology·Stanley Plotkin
Oct 20, 2017·Clinical and Vaccine Immunology : CVI·Mark R SchleissStanley A Plotkin
Aug 9, 2020·Frontiers in Microbiology·Yu-Qing Wang, Xiang-Yu Zhao
Nov 25, 2016·Drugs·K M AnderholmM R Schleiss

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