PMID: 20631436Jul 16, 2010Paper

Optimized surface-enhanced Raman scattering (SERS) colloids for the characterization of microorganisms

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Maria KnauerChristoph Haisch

Abstract

We report on a comparison of silver colloid preparation methods for the in situ analysis of microorganisms based on surface-enhanced Raman scattering (SERS). Different colloid sols were tested and optimized regarding their suitability as SERS substrates. The silver sols produced by a modified procedure of Leopold and Lendl gave an enhancement factor of the Raman signal in the order 10(8) for the test molecule, crystal violet. Furthermore, this SERS substrate was successfully applied for the in situ detection and identification of microorganisms immobilized on an immunoassay. The colloid preparation was carried out at room temperature and the colloids were stable for weeks. This silver nanoparticle preparation method for the label-free in situ detection of microorganisms successfully combines SERS with immunoassays. Hence, it has a great potential for the high-throughput detection of biomolecules.

References

Nov 1, 2000·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Y S LiY Wang
Dec 28, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Luis M Liz-Marzán
Jul 1, 2006·Analytical Chemistry·Adam M SchwartzbergChad E Talley
Aug 26, 2006·Analytical and Bioanalytical Chemistry·Atanu SenguptaE James Davis
Oct 14, 2006·Analytical Chemistry·Douglas A StuartRichard P Van Duyne
Oct 9, 2007·Physics in Medicine and Biology·K ZellC Haisch
Jul 30, 2008·Analytical Chemistry·Roger M JarvisRoyston Goodacre
Nov 4, 2008·Analytical and Bioanalytical Chemistry·Natalia P IvlevaChristoph Haisch
Dec 31, 2008·Physical Review Letters·M A NoginovV A Podolskiy
Jan 22, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·M HarzJ Popp
Mar 7, 2009·Analytical and Bioanalytical Chemistry·Katherine A Willets
Dec 5, 2009·Analytical Chemistry·Xiangjiang LiuChristoph Haisch

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Citations

Sep 29, 2011·Analytical and Bioanalytical Chemistry·Maria KnauerChristoph Haisch
May 10, 2013·Analytical and Bioanalytical Chemistry·Kathrin SchwarzmeierChristoph Haisch
Apr 14, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shiho TokonamiTsutomu Nagaoka
Apr 22, 2015·Advanced Drug Delivery Reviews·Susanne PahlowJürgen Popp
Mar 15, 2016·Analytical and Bioanalytical Chemistry·Christoph Haisch
Jan 11, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hemanth NoothalapatiTatsuyuki Yamamoto
Jul 10, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Chen ZhangWu-Li-Ji Hasi
Feb 6, 2018·Reviews in Analytical Chemistry·Anh H NguyenZachary D Schultz
Aug 16, 2017·Analytical and Bioanalytical Chemistry·Alexandra C WiesheuNatalia P Ivleva
Jan 4, 2021·Analytical and Bioanalytical Chemistry·Cristina-Cassiana AndreiAnne Chantal Gouget-Laemmel
Sep 4, 2020·Critical Reviews in Food Science and Nutrition·Hongbin PuXiaoli Li
Aug 6, 2010·The Journal of Physical Chemistry. B·Natalia P IvlevaChristoph Haisch
Jan 7, 2014·Analytical Chemistry·Haibo ZhouChristoph Haisch

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