Optimizing an Injectable Composite Oxygen-Generating System for Relieving Tissue Hypoxia

Frontiers in Bioengineering and Biotechnology
Tai-En HsiehChieh-Cheng Huang

Abstract

Oxygen deficiency resulting from bone fracture-induced vascular disruption leads to massive cell death and delayed osteoblast differentiation, ultimately impairing new bone formation and fracture healing. Enhancing local tissue oxygenation can help promote bone regeneration. In this work, an injectable composite oxygen-generating system consisting of calcium peroxide (CaO2)/manganese dioxide (MnO2)-encapsulated poly lactic-co-glycolic acid (PLGA) microparticles (CaO2 + MnO2@PLGA MPs) is proposed for the local delivery of oxygen. By utilizing a series of methodologies, the impacts of each component used for MP fabrication on the oxygen release behavior and cytotoxicity of the CaO2 + MnO2@ PLGA MPs are thoroughly investigated. Our analytical data obtained from in vitro studies indicate that the optimized CaO2 + MnO2@PLGA MPs developed in this study can effectively relieve the hypoxia of preosteoblast MC3T3-E1 cells that are grown under low oxygen tension and promote their osteogenic differentiation, thus holding great promise in enhancing fractural healing by increasing tissue oxygenation.

References

Jul 21, 2004·The Journal of Biological Chemistry·Ali SalimMichael T Longaker
Aug 8, 2007·Biomaterials·Benjamin S HarrisonJames J Yoo
Nov 21, 2008·Biomaterials·Se Heang OhBenjamin S Harrison
Dec 16, 2010·ACS Applied Materials & Interfaces·Junping WangXiaojun Yu
Feb 2, 2012·Nature Reviews. Rheumatology·Lutz ClaesAnita Ignatius
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eileen PedrazaCherie L Stabler
Mar 28, 2012·Nature Reviews. Rheumatology·Christa MaesErnestina Schipani
May 4, 2012·Science Translational Medicine·Suzanne ZeitouniCarl A Gregory
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Oct 16, 2012·Bone·Chuanyong LuRalph S Marcucio
Mar 5, 2014·International Journal of Molecular Sciences·Piergiorgio GentilePaul V Hatton
Aug 6, 2014·Bone·Enrique Gómez-BarrenaFlorian Gerbhard
Jun 9, 2015·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Prafulla K ChandraBenjamin S Harrison
Jul 15, 2015·Journal of Materials Science. Materials in Medicine·Hilde StegRoel Kuijer
Apr 15, 2016·ACS Applied Materials & Interfaces·Arun Kumar TeotiaAshok Kumar
Jun 22, 2016·Trends in Biotechnology·Mazaher GholipourmalekabadiAlexander M Seifalian
Jul 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ashley L FarrisWarren L Grayson
Sep 13, 2016·Scientific Reports·Zong-Keng KuoChao-Min Cheng
Nov 1, 2016·Scientific Reports·Jose M AyusoIgnacio Ochoa
Mar 28, 2017·Biomaterials·Maria M CoronelCherie L Stabler
Jun 7, 2017·Advanced Healthcare Materials·Justine C Lee, Elizabeth J Volpicelli
Aug 25, 2018·Biomaterials·Sujin Park, Kyung Min Park
Oct 27, 2018·ACS Applied Materials & Interfaces·Sang Min ParkDong Sung Kim

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