Optimizing and extending light-sculpting microscopy for fast functional imaging in neuroscience

Biomedical Optics Express
Peter RupprechtAlipasha Vaziri

Abstract

A number of questions in system biology such as understanding how dynamics of neuronal networks are related to brain function require the ability to capture the functional dynamics of large cellular populations at high speed. Recently, this has driven the development of a number of parallel and high speed imaging techniques such as light-sculpting microscopy, which has been used to capture neuronal dynamics at the whole brain and single cell level in small model organisms. However, the broader applicability of light-sculpting microcopy is limited by the size of volumes for which high speed imaging can be obtained and scattering in brain tissue. Here, we present strategies for optimizing the present tradeoffs in light-sculpting microscopy. Various scanning modalities in light-sculpting microscopy are theoretically and experimentally evaluated, and strategies to maximize the obtainable volume speeds, and depth penetration in brain tissue using different laser systems are provided. Design-choices, important parameters and their trade-offs are experimentally demonstrated by performing calcium-imaging in acute mouse-brain slices. We further show that synchronization of line-scanning techniques with rolling-shutter read-out of the ca...Continue Reading

References

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Citations

May 2, 2018·Annual Review of Neuroscience·Siegfried Weisenburger, Alipasha Vaziri
Oct 20, 2018·Biomedical Optics Express·Peter QuickeAmanda J Foust
Oct 31, 2017·Biomedical Optics Express·Mantas ŽurauskasMartin J Booth
Dec 1, 2019·Nanophotonics·Jialei TangKyu Young Han

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