Optimizing Biomaterials for Tissue Engineering Human Bone Using Mesenchymal Stem Cells

Plastic and Reconstructive Surgery
Christian WeinandJoseph P Vacanti

Abstract

Adequate biomaterials for tissue engineering bone and replacement of bone in clinical settings are still being developed. Previously, the combination of mesenchymal stem cells in hydrogels and calcium-based biomaterials in both in vitro and in vivo experiments has shown promising results. However, results may be optimized by careful selection of the material combination. β-Tricalcium phosphate scaffolds were three-dimensionally printed with five different hydrogels: collagen I, gelatin, fibrin glue, alginate, and Pluronic F-127. The scaffolds had eight channels, running throughout the entire scaffold, and macropores. Mesenchymal stem cells (2 × 10) were mixed with each hydrogel, and cell/hydrogel mixes were dispersed onto the corresponding β-tricalcium phosphate/hydrogel scaffold and cultured under dynamic-oscillating conditions for 6 weeks. Specimens were harvested at 1, 2, 4, and 6 weeks and evaluated histologically, radiologically, biomechanically and, at 6 weeks, for expression of bone-specific proteins by reverse-transcriptase polymerase chain reaction. Statistical correlation analysis was performed between radiologic densities in Hounsfield units and biomechanical stiffness. Collagen I samples had superior bone formation ...Continue Reading

References

May 1, 1991·Journal of Biomechanical Engineering·L G CimaR Langer
Jul 1, 1995·Medical Engineering & Physics·J Y RhoR B Ashman
Jun 1, 1995·The Journal of Bone and Joint Surgery. American Volume·T A Einhorn
Nov 3, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y JiangH K Genant
Apr 2, 1999·Science·M F PittengerD R Marshak
Jan 1, 1995·The Journal of the American Academy of Orthopaedic Surgeons·A R GazdagR A Forster
Aug 3, 2000·Journal of Biomechanical Engineering·B D PorterM J Yaszemski
Sep 12, 2000·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·F KorkusuzP Korkusuz
Mar 16, 2002·Journal of Periodontology·Edward B FowlerMichael A Billman
Jun 18, 2003·Biomaterials·L WangJ E Barralet
Feb 18, 2004·Annals of Biomedical Engineering·Lorenz MeinelGordana Vunjak-Novakovic
Jul 22, 2004·Tissue Engineering·Michelle R Kreke, Aaron S Goldstein
Dec 14, 2004·Tissue Engineering·M A LawsonJames T Triffitt
Dec 27, 2005·Bone·Christian WeinandJoseph P Vacanti
Dec 26, 2006·Journal of Bioscience and Bioengineering·Akira TachibanaKiyoshi Yamauchi
Apr 24, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Christian WeinandThomas J Gill
Jan 1, 2013·Organogenesis·Ge Zhang
Jan 3, 2013·Advances in Experimental Medicine and Biology·Josh NemanRahul Jandial
Jan 4, 2013·Tissue Engineering. Part C, Methods·Alex J LomasNicholas R Forsyth
Jan 4, 2013·Annals of Biomedical Engineering·Qingsu ChengEhsan Jabbarzadeh
Jan 10, 2013·Trends in Biotechnology·Kim E M BendersJos Malda
May 8, 2014·Journal of Cellular and Molecular Medicine·Raymund E HorchAndreas Arkudas

❮ Previous
Next ❯

Citations

Feb 22, 2017·Drug Discovery Today·Bapi GorainRakesh Kumar Tekade
Feb 15, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Marek KaweckiAleksandra Kasperczyk
Apr 26, 2018·International Journal of Molecular Sciences·Leandra Santos BaptistaJosé Mauro Granjeiro
Feb 13, 2018·The Journal of Surgical Research·Christopher D LopezPaulo G Coelho
May 9, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Wuwei LiBo Wang
May 31, 2021·International Journal of Biological Macromolecules·Fei XingChenguang Liu
Nov 28, 2017·Biomacromolecules·Supachai Reakasame, Aldo R Boccaccini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.