Optimizing methods for human testicular tissue cryopreservation and spermatogonial stem cell isolation

Journal of Cellular Biochemistry
Seyedeh-Faezeh MoravejiHossein Baharvand

Abstract

Cryopreservation of testicular tissue before cancer therapy for fertility preservation in prepubertal boys with cancer is of great interest in reproductive medicine. Isolation of spermatogonial stem cells (SSCs) from cryopreserved tissues would be a suitable cell source to re-establish spermatogenesis after cancer therapy. We herein establish optimized protocols for cryopreservation of human testicular tissue and isolation of SSCs from cryopreserved tissue. We developed a freezing protocol that provided high testicular cell viability and supported structural integrity and tubular epithelium coherence similar to fresh tissue. Then, we established a protocol that allowed efficient isolation of functional SSCs from cryopreserved tissues. Isolated cells were found on the testicular basement membrane after xenotransplantation. Our results demonstrated the preservation of testicular tissue structure and high cell viability with efficient isolation of SSCs after testicular cryopreservation, which is promising for future therapeutic applications in fertility preservation.

References

Aug 26, 1998·Molecular Reproduction and Development·G VajtaH Callesen
Feb 10, 2009·Journal of Assisted Reproduction and Genetics·Firooz Jannat AlipoorMehdi Lotfi Panah
Nov 19, 2009·JAMA : the Journal of the American Medical Association·Hooman Sadri-ArdekaniAns M M van Pelt
Jun 15, 2010·Fertility and Sterility·Mara CurabaJacques Donnez
Feb 9, 2011·Fertility and Sterility·Mara CurabaChristine Wyns
Jan 15, 2013·Human Reproduction·Jonathan PoelsChristine Wyns
Sep 10, 2013·Fertility and Sterility·Marvin L Meistrich
Dec 29, 2015·Reproductive Medicine and Biology·Tetsuhiro Yokonishi, Takehiko Ogawa
Feb 27, 2016·Methods : a Companion to Methods in Enzymology·Hooman Sadri-ArdekaniAnthony Atala

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Citations

Nov 2, 2019·International Journal of Molecular Sciences·Lihua DongClaus Yding Andersen
Dec 5, 2019·Clinical Medicine Insights. Reproductive Health·Aude BrayeEllen Goossens
Apr 2, 2021·Cell and Tissue Research·Tanushree PatraMukesh Kumar Gupta
Aug 11, 2020·The International Journal of Biochemistry & Cell Biology·Azam JavadiFereshteh Esfandiari

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