Optimizing outpatient serial sputum colony counting for studies of tuberculosis treatment in resource-poor settings.

Journal of Clinical Microbiology
Derek J SloanGeraint R Davies

Abstract

Serial Sputum Colony Counting (SSCC) is an important technique in clinical trials of new treatments for tuberculosis (TB). Quantitative cultures on selective Middlebrook agar are used to calculate the rate of bacillary elimination from sputum collected from patients at different time points during the first 2 months of therapy. However, the procedure can be complicated by high sample contamination rates. This study, conducted in a resource-poor setting in Malawi, assessed the ability of different antifungal drugs in selective agar to reduce contamination. Overall, 229 samples were studied and 15% to 27% were contaminated. Fungal organisms were particularly implicated, and samples collected later in treatment were at particular risk (P < 0.001). Amphotericin B (AmB) is the standard antifungal drug used on SSCC plates at a concentration of 10 mg/ml. On selective Middlebrook 7H10 plates, AmB at 30 mg/ml reduced sample contamination by 17% compared with AmB at 10 mg/ml. The relative risk of contamination using AmB at 10 mg/ml was 1.79 (95% confidence interval [CI], 1.25 to 3.55). On Middlebrook 7H11 plates, a combination of AmB at 10 mg/ml and carbendazim at 50 mg/ml was associated with 10% less contamination than AmB at 30 mg/ml. ...Continue Reading

References

May 1, 1972·Journal of Medical Microbiology·D A MitchisonV R Aber
Apr 1, 1973·Journal of Clinical Pathology·D A MitchisonR A Lambert
Dec 1, 1973·Antimicrobial Agents and Chemotherapy·E R Block, J E Bennett
Jan 1, 1981·Journal of Clinical Microbiology·M V RothlaufE B Blair
Dec 1, 1993·The American Review of Respiratory Disease·N KennedyS H Gillespie
May 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·N KennedyS H Gillespie
Jun 13, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·H F ChambersP C Hopewell
Jul 21, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mohamed Abdel Aziz, Abigail Wright
Jul 15, 2006·The Journal of Antimicrobial Chemotherapy·D A Mitchison
Jul 22, 2006·The Journal of Antimicrobial Chemotherapy·G R DaviesL J Aarons
Jul 27, 2006·PLoS Medicine·Joshua A SalomonMartien W Borgdorff
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Geraint R DaviesLeon J Aarons
Jun 6, 2009·The New England Journal of Medicine·Andreas H DiaconDavid F Mc Neeley
Aug 30, 2011·Ecotoxicology and Environmental Safety·Xiuguo WangYunlong Yu
Jan 16, 2013·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·E L MacielJ L Johnson

❮ Previous
Next ❯

Citations

Jan 7, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sandeep TyagiJacques H Grosset
Nov 15, 2015·Trials·Laura Jayne Bonnett, Geraint Rhys Davies
Mar 18, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Derek J SloanGeraint R Davies
Dec 18, 2018·Nanoscale·Oskar FranchBirgitta Ruth Knudsen
Mar 2, 2019·The Journal of Antimicrobial Chemotherapy·Devyani DeshpandeTawanda Gumbo
Jan 8, 2014·Expert Review of Anti-infective Therapy·Andreas H Diacon, Peter R Donald
Feb 26, 2019·Frontiers in Pediatrics·Mohamed I M AhmedUlrich von Both
Jul 22, 2018·Pharmaceutical Statistics·Divan Aristo BurgerDing-Geng Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Related Papers

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
A H DiaconP R Donald
Antimicrobial Agents and Chemotherapy
E C Delaha, V F Garagusi
© 2021 Meta ULC. All rights reserved