Abstract
Serial Sputum Colony Counting (SSCC) is an important technique in clinical trials of new treatments for tuberculosis (TB). Quantitative cultures on selective Middlebrook agar are used to calculate the rate of bacillary elimination from sputum collected from patients at different time points during the first 2 months of therapy. However, the procedure can be complicated by high sample contamination rates. This study, conducted in a resource-poor setting in Malawi, assessed the ability of different antifungal drugs in selective agar to reduce contamination. Overall, 229 samples were studied and 15% to 27% were contaminated. Fungal organisms were particularly implicated, and samples collected later in treatment were at particular risk (P < 0.001). Amphotericin B (AmB) is the standard antifungal drug used on SSCC plates at a concentration of 10 mg/ml. On selective Middlebrook 7H10 plates, AmB at 30 mg/ml reduced sample contamination by 17% compared with AmB at 10 mg/ml. The relative risk of contamination using AmB at 10 mg/ml was 1.79 (95% confidence interval [CI], 1.25 to 3.55). On Middlebrook 7H11 plates, a combination of AmB at 10 mg/ml and carbendazim at 50 mg/ml was associated with 10% less contamination than AmB at 30 mg/ml. ...Continue Reading
References
May 1, 1972·Journal of Medical Microbiology·D A MitchisonV R Aber
Apr 1, 1973·Journal of Clinical Pathology·D A MitchisonR A Lambert
Dec 1, 1973·Antimicrobial Agents and Chemotherapy·E R Block, J E Bennett
Jan 1, 1981·Journal of Clinical Microbiology·M V RothlaufE B Blair
Dec 1, 1993·The American Review of Respiratory Disease·N KennedyS H Gillespie
May 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·N KennedyS H Gillespie
Jun 13, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·H F ChambersP C Hopewell
Dec 12, 2001·BMC Pulmonary Medicine·R BrindleD Mitchison
Jul 21, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mohamed Abdel Aziz, Abigail Wright
Feb 7, 2006·Breast Cancer Research : BCR·Toral GathaniUNKNOWN Million Women Study Collaborators
Jul 15, 2006·The Journal of Antimicrobial Chemotherapy·D A Mitchison
Jul 22, 2006·The Journal of Antimicrobial Chemotherapy·G R DaviesL J Aarons
Jul 27, 2006·PLoS Medicine·Joshua A SalomonMartien W Borgdorff
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Geraint R DaviesLeon J Aarons
May 2, 2008·Journal of Clinical Microbiology·Louis GrandjeanCarlton A Evans
May 29, 2008·Antimicrobial Agents and Chemotherapy·R RustomjeeD F McNeeley
Apr 7, 2009·Lancet·Marcus B CondeRichard E Chaisson
Jun 6, 2009·The New England Journal of Medicine·Andreas H DiaconDavid F Mc Neeley
Aug 30, 2011·Ecotoxicology and Environmental Safety·Xiuguo WangYunlong Yu
Jul 20, 2012·Tropical Medicine & International Health : TM & IH·C FeldackerS Phiri
Jan 16, 2013·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·E L MacielJ L Johnson
Citations
Jan 7, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sandeep TyagiJacques H Grosset
May 10, 2016·Tuberculosis·David A BarrDerek J Sloan
Nov 15, 2015·Trials·Laura Jayne Bonnett, Geraint Rhys Davies
Mar 18, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Derek J SloanGeraint R Davies
Jul 28, 2015·PloS One·Benjawan KaewseekhaoKiatichai Faksri
Feb 18, 2016·PloS One·Cristina BotellaRosa María Baños
Dec 18, 2018·Nanoscale·Oskar FranchBirgitta Ruth Knudsen
Mar 2, 2019·The Journal of Antimicrobial Chemotherapy·Devyani DeshpandeTawanda Gumbo
May 1, 2019·PloS One·Sumona DattaCarlton A Evans
Jan 8, 2014·Expert Review of Anti-infective Therapy·Andreas H Diacon, Peter R Donald
Oct 17, 2018·Frontiers in Immunology·Mohamed I M AhmedChristof Geldmacher
Aug 1, 2017·International Journal of Pharmacokinetics·Andrew D McCallum, Derek J Sloan
Nov 7, 2017·Statistics in Medicine·Divan Aristo Burger, Robert Schall
Feb 26, 2019·Frontiers in Pediatrics·Mohamed I M AhmedUlrich von Both
Jul 22, 2018·Pharmaceutical Statistics·Divan Aristo BurgerDing-Geng Chen