Optimizing selection of large animals for antibody production by screening immune response to standard vaccines

Journal of Immunological Methods
Mary K ThompsonMichael P Rout

Abstract

Antibodies made in large animals are integral to many biomedical research endeavors. Domesticated herd animals like goats, sheep, donkeys, horses and camelids all offer distinct advantages in antibody production. However, their cost of use is often prohibitive, especially where poor antigen response is commonplace; choosing a non-responsive animal can set a research program back or even prevent experiments from moving forward entirely. Over the course of production of antibodies from llamas, we found that some animals consistently produced a higher humoral antibody response than others, even to highly divergent antigens, as well as to their standard vaccines. Based on our initial data, we propose that these "high level responders" could be pre-selected by checking antibody titers against common vaccines given to domestic farm animals. Thus, time and money can be saved by reducing the chances of getting poor responding animals and minimizing the use of superfluous animals.

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Nov 5, 2014·Nature Methods·Peter C FridyMichael P Rout

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Citations

Jan 15, 2017·Methods : a Companion to Methods in Enzymology·Philippe Delahaut
Sep 6, 2018·Biomédica : revista del Instituto Nacional de Salud·Eduardo EgeaLuis Alejandro Barrera
Mar 28, 2019·Combinatorial Chemistry & High Throughput Screening·Jane Ru ChoiAlistair C Cowie
May 4, 2021·Frontiers in Microbiology·Julio FloresLeslie Chavez-Galan

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