Optimizing stem cell functions and antibacterial properties of TiO2 nanotubes incorporated with ZnO nanoparticles: experiments and modeling

International Journal of Nanomedicine
Wenwen LiuThomas J Webster

Abstract

To optimize mesenchymal stem cell differentiation and antibacterial properties of titanium (Ti), nano-sized zinc oxide (ZnO) particles with tunable concentrations were incorporated into TiO2 nanotubes (TNTs) using a facile hydrothermal strategy. It is revealed here for the first time that the TNTs incorporated with ZnO nanoparticles exhibited better biocompatibility compared with pure Ti samples (controls) and that the amount of ZnO (tailored by the concentration of Zn(NO3)2 in the precursor) introduced into TNTs played a crucial role on their osteogenic properties. Not only was the alkaline phosphatase activity improved to about 13.8 U/g protein, but the osterix, collagen-I, and osteocalcin gene expressions was improved from mesenchymal stem cells compared to controls. To further explore the mechanism of TNTs decorated with ZnO on cell functions, a response surface mathematical model was used to optimize the concentration of ZnO incorporation into the Ti nanotubes for stem cell differentiation and antibacterial properties for the first time. Both experimental and modeling results confirmed (R (2) values of 0.8873-0.9138 and 0.9596-0.9941, respectively) that Ti incorporated with appropriate concentrations (with an initial conce...Continue Reading

Citations

May 19, 2017·Nanomedicine·Joseph S Fernandez-MoureEnnio Tasciotti
May 12, 2019·Journal of Biomedical Materials Research. Part a·Amin RahmaniForough Azam Sayahpour
Feb 1, 2020·Journal of Biomaterials Applications·Shang GaoSu Chen
Apr 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yan ChengYuekun Lai
Jun 8, 2017·Journal of Applied Toxicology : JAT·Hong SuMin Ding
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Jianqiao LiuYujin Tang
Nov 30, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Hongyan ChenMeifang Zhu

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Methods Mentioned

BETA
scanning
atomic
transmission electron microscopy
X-ray
profiler
Protein Assay
atomic force microscopy
scanning electron microscopy

Software Mentioned

Design
- Expert

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