Optimizing Temperature and Oxygen Supports Long-term Culture of Human Islets

Transplantation
H KomatsuY Mullen

Abstract

Islet transplantation is a promising treatment for type-1 diabetes; however, donor shortage is a concern. Even when a pancreas is available, low islet yield limits the success of transplantation. Islet culture enables pooling of multiple low-yield isolations into an effective islet mass, but isolated islets rapidly deteriorate under conventional culture conditions. Oxygen (O2) depletion in the islet core, which leads to central necrosis and volume loss, is one of the major reasons for this deterioration. To promote long-term culture of human islets in PIM-R medium (used for islet research), we adjusted temperature (12°C, 22°C, and 37°C) and O2 concentration (21% and 50%). We simulated the O2 distribution in islets based on islet O2 consumption rate and dissolved O2 in the medium. We determined the optimal conditions for O2 distribution and volume maintenance in a 2-week culture and assessed viability and insulin secretion compared to noncultured islets. In vivo islet engraftment was assessed by transplantation into diabetic nonobese diabetic-severe combined immunodeficiency mouse kidneys. We validated our results using CMRL 1066 medium (used for clinical islet transplantation). Simulation revealed that 12°C of 50% O2 PIM-R cult...Continue Reading

References

Nov 1, 1978·The American Journal of Physiology·J S WillisJ H Becker
Jul 1, 1983·Diabetologia·L Jansson, C Hellerström
Jan 1, 1995·Annual Review of Medicine·J H Southard, F O Belzer
Jan 1, 1993·Renal Physiology and Biochemistry·F LangD Häussinger
Jan 1, 1997·Transplant International : Official Journal of the European Society for Organ Transplantation·P K VreugdenhilJ H Southard
Jun 9, 1998·Annals of the New York Academy of Sciences·E S Avgoustiniatos, C K Colton
Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K VajdováM Lutterová
Jun 1, 2006·Acta Physiologica·M I PanayiotidisJ A Cidlowski
Mar 3, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K OmoriY Mullen
Apr 18, 2009·Theoretical Biology & Medical Modelling·Peter Buchwald
Jan 26, 2010·Transplantation·Heide BrandhorstDaniel Brandhorst
Nov 26, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Christian PflegerMichael Eckhard
Jan 13, 2012·Cell Transplantation·Itzia IglesiasIsmail H Al-Abdullah
Feb 11, 2014·Diabetes & Metabolism·M-C VantyghemP F Westeel
Apr 8, 2014·The International Journal of Biochemistry & Cell Biology·Laura TerraneoMichele Samaja
May 31, 2014·Journal of Diabetes Science and Technology·Thomas M SuszynskiKlearchos K Papas
Jun 10, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·N M Luan, H Iwata
Jun 20, 2014·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Fei LiuZhi Qi
May 2, 2015·Diabetologia·Jocelyn E Manning FoxPatrick E MacDonald
Jan 24, 2016·Biochemical and Biophysical Research Communications·Hirotake KomatsuYoko Mullen
Apr 28, 2016·American Journal of Physiology. Endocrinology and Metabolism·Keiko OmoriYoko Mullen
Sep 3, 2016·Cryobiology·Keiko OmoriYoko Mullen
Nov 23, 2016·Journal of Diabetes Research·Thomas M SuszynskiKlearchos K Papas
Mar 30, 2017·Biochemical and Biophysical Research Communications·Hirotake KomatsuYoko Mullen
Sep 1, 2004·Cell Transplantation·T B MurdochJ R T Lakey
Sep 13, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H KomatsuY Mullen
Jan 13, 2018·Annals of Transplantation : Quarterly of the Polish Transplantation Society·Matteo RavaioliAntonio D Pinna

❮ Previous
Next ❯

Citations

Mar 22, 2020·Transplant International : Official Journal of the European Society for Organ Transplantation·Hirotake KomatsuYoko Mullen
Apr 4, 2021·International Journal of Molecular Sciences·Fiona M Docherty, Lori Sussel
Oct 12, 2021·Cell Transplantation·Hirotake KomatsuIsmail H Al-Abdullah

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.