PMID: 6361161Jan 1, 1983Paper

Optimizing the lymphocyte transformation test in whole blood. I. Optimum conditions for thymidine incorporation

Journal of Immunopharmacology
U BickerV Haas


The correlation between mitogen concentration and H3-thymidine incorporation can be described mathematically by a sigmoid dose-response curve. Thymidine incorporation/lymphocyte (Ti/Ly) was calculated to facilitate comparison of results. It was highest at a blood dilution of 1:50, lower at 1:10 or 1:250. Ti/Ly obtained with optimum concentrations of PHA was higher than with ConA. Advantages of this optimized method for whole blood over Ficoll-separated mononuclear cells are the higher Ti/Ly, the smaller quantities of blood required and the lower experimental effort.


Jan 1, 1975·Immunological Communications·J EskolaO Ruuskanen
Jan 1, 1976·International Archives of Allergy and Applied Immunology·D E BiceJ Salvaggio
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·B H Park, R A Good
Feb 1, 1972·Cellular Immunology·R A JohnsonC H Kirkpatrick


Aug 18, 1995·Journal of Immunological Methods·A A Fasanmade, W J Jusko
Jan 1, 1985·Journal of Immunopharmacology·E SchlickM A Chirigos
Jan 1, 1984·Journal of Immunopharmacology·J J ReiterU Bicker

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