Abstract
Fine-tuning loading and release of therapeutic and imaging agents associated with polymeric matrices is a fundamental step in the preclinical development of novel nanomedicines. Here, 1,000 × 400 nm Discoidal Polymeric Nanoconstructs (DPNs) were realized via a top-down, template-based fabrication approach, mixing together poly(lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol)-diacrylate (PEG-DA) chains in a single polymer paste. Two different loading strategies were tested, namely the "direct loading" and the "absorption loading." In the first case, the agent was directly mixed with the polymeric paste to realize DPNs whereas, in the second case, DPNs were first lyophilized and then rehydrated upon exposure to a concentrated aqueous solution of the agent. Under these two loading conditions, the encapsulation efficiencies and release profiles of different agents were systematically assessed. Specifically, six agents were realized by conjugating lipid chains (DSPE) or polymeric chains (PEG) to the near-infrared imaging molecule Cy5 (DSPE-Cy5 A and DSPE-Cy5 B); the chemotherapeutic molecules methotrexate (DSPE-MTX and PEG-MTX) and doxorubicin (LA-DOX and DSPE-DOX). Moderately hydrophobic compounds with low molecular weight...Continue Reading
References
Mar 4, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·H MaedaK Hori
Apr 3, 2008·Small·Jie LuJeffrey I Zink
Jun 3, 2008·Advanced Drug Delivery Reviews·Igor I SlowingVictor S-Y Lin
Jun 10, 2008·Nano Letters·Ziyang HuoPeidong Yang
Jul 26, 2008·Nature Nanotechnology·Dan PeerRobert Langer
Apr 9, 2009·Journal of the American Chemical Society·Sarit S AgastiVincent M Rotello
Apr 25, 2013·Biomaterials·Jaehong KeyPaolo Decuzzi
Dec 21, 2013·Nanomedicine·Randall ToyEfstathios Karathanasis
Feb 14, 2014·Journal of Pharmaceutical Sciences·Takayuki KatsubeYoshitaka Yano
Oct 22, 2015·ACS Nano·Jaehong KeyPaolo Decuzzi
Jan 23, 2016·Chemical Reviews·Guanying ChenParas N Prasad
Jun 15, 2016·Pharmaceutical Research·Daniel BoboSimon R Corrie
Nov 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Renata S FernandesAndré L B de Barros
Mar 28, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Anna Lisa PalangePaolo Decuzzi
Jul 4, 2017·Journal of Pharmaceutical Sciences·Az Alddien NatfjiFrancesca Greco
Nov 8, 2017·Theranostics·Aeju LeePaolo Decuzzi
Jan 10, 2018·ACS Nano·Roberto PalombaPaolo Decuzzi
Mar 27, 2018·Journal of Biomedical Materials Research. Part a·Prachi DesaiAkhilesh K Gaharwar
Aug 15, 2018·BMC Cancer·Bray DenardJin Ye
Jan 11, 2019·Oncology Reports·Chyou-Wei WeiGiou-Teng Yiang
May 10, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kinam ParkYan Wang
Jan 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pengfei GouMeihua Sui
Citations
Apr 4, 2021·Pharmaceutics·Valentina Di FrancescoMiguel Ferreira
Jun 3, 2021·International Journal of Molecular Sciences·Gemma Di PompoSofia Avnet
Jul 2, 2021·ACS Applied Materials & Interfaces·Martina Di FrancescoPaolo Decuzzi
Aug 19, 2020·ACS Applied Materials & Interfaces·Valentina Di FrancescoPaolo Decuzzi