Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures

Scientific Reports
June Hoan KimWoong Sun

Abstract

Tissue-clearing techniques have received great attention for volume imaging and for the potential to be applied in optical diagnosis. In principle, tissue clearing is achieved by reducing light scattering through a combination of lipid removal, size change, and matching of the refractive index (RI) between the imaging solution and the tissue. However, the contributions of these major factors in tissue clearing have not been systematically evaluated yet. In this study, we experimentally measured and mathematically calculated the contribution of these factors to the clearing of four organs (brain, liver, kidney, and lung). We found that these factors differentially influence the maximal clearing efficacy of tissues and the diffusivity of materials inside the tissue. We propose that these physical properties of organs can be utilized for the quality control (Q/C) process during tissue clearing, as well as for the monitoring of the pathological changes of tissues.

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·E D LaywellD A Steindler
Aug 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A López-De León, M Rojkind
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cindy Y JaoAdrian Salic
Jan 21, 2011·Nature Reviews. Neuroscience·Stefanie RobelMagdalena Götz
Oct 13, 2012·Nature Protocols·Ali ErtürkHans-Ulrich Dodt
Apr 12, 2013·Nature·Kwanghun ChungKarl Deisseroth
Dec 19, 2013·Laser & Photonics Reviews·Dan ZhuValery V Tuchin
Jun 20, 2014·Nature Protocols·Raju TomerKarl Deisseroth
Mar 12, 2015·Journal of Biophotonics·Daria K TuchinaValery V Tuchin
Apr 2, 2015·Experimental Neurology·Joshua E BurdaMichael V Sofroniew
Jul 18, 2015·Cell·Douglas S Richardson, Jeff W Lichtman
Aug 23, 2016·Nature Methods·Chenchen PanAli Ertürk
Sep 7, 2016·Scientific Reports·Kristian H R Jensen, Rune W Berg
Feb 28, 2017·Journal of Biomedical Optics·Sónia CarvalhoValery V Tuchin
Apr 1, 2017·Nature Methods·Rory M Power, Jan Huisken
Apr 19, 2017·Nature Methods·Jae-Byum ChangEdward S Boyden
Jul 7, 2017·Cell Reports·Shimpei I KubotaHiroki R Ueda
Aug 3, 2017·Journal of Biophotonics·Luís M OliveiraValery V Tuchin
Oct 1, 2017·Nature Biomedical Engineering·Nobuyuki TanakaPer Uhlén

❮ Previous
Next ❯

Citations

Jun 14, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Cinzia BrennaNorbert Gretz
Jul 21, 2020·Journal of Biophotonics·Daria K TuchinaValery V Tuchin
Sep 18, 2020·Journal of Anatomy·Ting TianXiaoguang Li
Apr 25, 2020·Advanced Science·Jingtan ZhuDan Zhu
Feb 17, 2021·Methods and Applications in Fluorescence·Sergiy V Avilov
Mar 12, 2021·Bioinformatics·Friedrich PreusserStephan Preibisch
Jul 2, 2021·Frontiers in Cell and Developmental Biology·Etsuo A Susaki, Minoru Takasato
Aug 4, 2021·Journal of Cell Science·Julian Hofmann, Selina J Keppler

❮ Previous
Next ❯

Methods Mentioned

BETA
light scattering
fluorescence imaging

Software Mentioned

iDISCO
DISCO
CLARITY
CUBIC
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.