Abstract
Cumulus-free in vitro maturation (IVM) provides a powerful tool to manipulate immature oocytes, but IVM oocytes lead to poor development after fertilization. Supplementation of the culture medium with tauroursodeoxycholic acid (TUDCA), a bile acid, has been reported to improve the development of embryos derived from in vivo fertilized (IVF) embryos after in vitro culture (IVC) by attenuating endoplasmic reticulum stress. However, it remains unclear if TUDCA can improve development of IVM-IVF embryos. Here, we examined whether TUDCA treatment could improve embryonic development during or after IVM. Immature GV oocytes collected from ovaries of ICR female mice that were free from cumulus cells were subjected to IVM in αMEM containing 5% FBS for 16 h. TUDCA was added to the media at varying concentrations (0-1000 μM) during IVM and IVC. TUDCA treatment during IVM reduced both MII and pronuclear (PN) rates but did not affect blastocyst rates of fertilized embryos. In contrast, TUDCA treatment during IVC significantly increased blastocyst formation rates in a concentration dependent manner. Finally, embryo transfer after TUDCA treatment revealed a significant improvement in the rates of offspring production (15% with 1000 μM TUDCA v...Continue Reading
References
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·A OguraJ Matsuda
Jan 5, 2002·Zygote : the Biology of Gametes and Early Embryos·Y YamazakiR Yanagimachi
Oct 18, 2002·Obstetrics and Gynecology·Tim J ChildSeang Lin Tan
Jul 17, 2004·Journal of Neurochemistry·Rita M RamalhoCecília M P Rodrigues
Dec 17, 2005·Biochemical and Biophysical Research Communications·Satoshi KishigamiTeruhiko Wakayama
Jan 18, 2006·The Journal of Reproduction and Development·Hiromi MikiAtsuo Ogura
Nov 10, 2006·Theriogenology·Robert B Gilchrist, Jeremy G Thompson
Jan 20, 2009·Human Reproduction·J J EppigB A King
May 7, 2009·Journal of Lipid Research·Joana D AmaralCecília M P Rodrigues
Mar 19, 2011·Biology of Reproduction·Azusa InoueFugaku Aoki
Jul 19, 2012·PloS One·Jin Yu ZhangDong Il Jin
Aug 31, 2013·Nature Reviews. Drug Discovery·Claudio HetzHeather P Harding
Oct 18, 2013·Biology of Reproduction·Masatoshi OogaFugaku Aoki
Aug 12, 2014·World Journal of Gastroenterology : WJG·David Q-H Wang, Martin C Carey
Jun 9, 2015·Reproductive Biology·Tao LinDong Il Jin
Oct 25, 2016·Molecular Neurobiology·Inês FonsecaMargarida Castro-Caldas
Nov 4, 2017·PloS One·Naomi DicksVilceu Bordignon
May 12, 2018·Toxicology Letters·Zhanwei JiaFengyi Li
Citations
Dec 6, 2019·Journal of Assisted Reproduction and Genetics·Tengfei DengCuilian Zhang
May 3, 2019·Balsaeng'gwa saengsig·Yeo-Reum ParkHee-Tae Cheong
May 16, 2020·Balsaeng'gwa saengsig·Hye-Bin ParkHee-Tae Cheong
Mar 9, 2021·Frontiers in Cell and Developmental Biology·Elisa Mariano PioltineMarcelo Fábio Gouveia Nogueira